國家衛生研究院 NHRI:Item 3990099045/6883

NHRI > Immunology Research Center > Ching-Liang Chu(2005-2010) > Conference Papers/Meeting Abstract > Item 3990099045/6883

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Title:	Increased TLR responses in dendritic cells lacking the ITAM-containing adapters DAP12 and FcRgamma
Other Titles:	Increased TLR Responses in Dendritic Cells Lacking the Itam-Containing Adapters Dap12 and FcRγ
Authors:	Hamerman, JA;Yu, YL;Shen, KY;Lowell, CA;Lanier, LL;Chu, CL
Contributors:	Immunology Research Center
Abstract:	The inhibitory effect of DAP12 on macrophages has been revealed by examining myeloid cells from DAP12-deficient mice. In this report, we demonstrate that both DAP12 and the FcϵRIγ-chain (FcRγ) are required for negative regulation of TLR responses in bone marrow-derived dendritic cells (DC). Loss of both DAP12 and FcRγ enhanced the pro-inflammatory cytokine production and maturation of DC after TLR stimulation, resulting in a greater percentage of DC that produced IL-12 p40, TNF, and IL-6, and expressed high levels of MHC class II, CD80, and CD86. Whereas DC lacking only DAP12 showed some increased TLR responses, those lacking only FcRγ had a greater enhancement of maturation and cytokine production, though to a lesser extent than DC lacking both DAP12 and FcRγ. Additionally, antigen-specific T cell proliferation was enhanced by DAP12–/–FcRγ–/– DC relative to wild-type DC after maturation. Similar to DAP12–/–FcRγ–/– DC, Syk-deficient DC also had increased inflammatory cytokine production, maturation, and antigen presentation. These results confirm the inhibitory effect of immunoreceptor tyrosine-based activation motif (ITAM) signaling in myeloid cells and show that DC and macrophages differ in their dependence on the ITAM-containing adapters DAP12 and FcRγ for negative regulation of TLR signaling.
Date:	2008-04
Relation:	FASEB Journal. 2008 Apr;22(Suppl.):1065.36.
Link to:	http://www.fasebj.org/cgi/content/meeting_abstract/22/1_MeetingAbstracts/1065.36
JIF/Ranking 2023:	http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0892-6638&DestApp=IC2JCR
Cited Times(WOS):	https://www.webofscience.com/wos/woscc/full-record/WOS:000208467808342
Appears in Collections:	[Ching-Liang Chu(2005-2010)] Conference Papers/Meeting Abstract

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