國家衛生研究院 NHRI:Item 3990099045/6844

NHRI > Institute of Biomedical Engineering and Nanomedicine > Chia-Hsien Hsu > Periodical Articles > Item 3990099045/6844

Please use this identifier to cite or link to this item: http://ir.nhri.org.tw/handle/3990099045/6844

Title:	AlGaN/GaN high electron mobility transistors for protein-peptide binding affinity study
Authors:	Huang, CC;Lee, GY;Chyi, JI;Cheng, HT;Hsu, CP;Hsu, YR;Hsu, CH;Huang, YF;Sun, YC;Chen, CC;Li, SS;Andrew Yeh, J;Yao, DJ;Ren, F;Wang, YL
Contributors:	Division of Medical Engineering Research
Abstract:	Antibody-immobilized AlGaN/GaN high electron mobility transistors (HEMTs) were used to detect a short peptide consisting of 20 amino acids. One-binding-site model and two-binding-site model were used for the analysis of the electrical signals, revealing the number of binding sites on an antibody and the dissociation constants between the antibody and the short peptide. In the binding-site models, the surface coverage ratio of the short peptide on the sensor surface is relevant to the electrical signals resulted from the peptide–antibody binding on the HEMTs. Two binding sites on an antibody were observed and two dissociation constants, 4.404×10−11 M and 1.596×10−9 M, were extracted from the binding-site model through the analysis of the surface coverage ratio of the short peptide on the sensor surface. We have also shown that the conventional method to extract the dissociation constant from the linear regression of curve-fitting with Langmuir isotherm equation may lead to an incorrect information if the receptor has more than one binding site for the ligand. The limit of detection (LOD) of the sensor observed in the experimental result (∼10 pM of the short peptide) is very close to the LOD (around 2.7–3.4 pM) predicted from the value of the smallest dissociation constants. The sensitivity of the sensor is not only dependent on the transistors, but also highly relies on the affinity of the ligand-receptor pair. The results demonstrate that the AlGaN/GaN HEMTs cannot only be used for biosensors, but also for the biological affinity study.
Date:	2013-03
Relation:	Biosensors and Bioelectronics. 2013 Mar;41:717-722.
Link to:	http://dx.doi.org/10.1016/j.bios.2012.09.066
JIF/Ranking 2023:	http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0956-5663&DestApp=IC2JCR
Cited Times(WOS):	https://www.webofscience.com/wos/woscc/full-record/WOS:000314191300107
Cited Times(Scopus):	http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84870823009
Appears in Collections:	[Chia-Hsien Hsu] Periodical Articles

Files in This Item:

File	Description	Size	Format
SDO0956566312006720.pdf		627Kb	Adobe PDF	508	View/Open

Loading...