國家衛生研究院 NHRI:Item 3990099045/6813
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    Please use this identifier to cite or link to this item: http://ir.nhri.org.tw/handle/3990099045/6813


    Title: Species identification of Wickerhamomyces anomalus and related taxa using Beta-tubulin (Beta-tub) DNA barcode marker
    Other Titles: Species identification of Wickerhamomyces anomalus and related taxa using B-tubulin (B-tub) DNA barcode marker
    Authors: Huang, CH;Chang, MT;Huang, L
    Contributors: Institute of Biotechnology and Pharmaceutical Research
    Abstract: Wickerhamomyces anomalus is used in food and feed processing, although the species has been reported as an opportunistic human pathogen, predominantly in neonates. Neither phenotypic nor the most frequently applied genotypic marker (D1/D2 LSU ribosomal DNA) provide sufficient resolution for accurate identification of this yeast. In this study, the β-tubulin gene was used for species identification by direct DNA sequencing and as marker in a species-specific PCR assay. The results showed that all examined W. anomalus strains were clearly distinguished from the closely related species by comparative sequence analysis of the β-tubulin gene. In addition, the species-specific primers were also developed based on the β-tubulin gene, which was employed for polymerase chain reaction with the template DNA of Wickerhamomyces strains. A single 218 bp species-specific band was found only in W. anomalus. Our data indicate that the phylogenetic relationships between these strains are easily resolved by sequencing of the β-tubulin gene and combined with species-specific PCR assay.
    Date: 2012-12
    Relation: Yeast. 2012 Dec;29(12):531-535.
    Link to: http://dx.doi.org/10.1002/yea.2933
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0749-503X&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000312539000004
    Cited Times(Scopus): https://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84871019893
    Appears in Collections:[Others] Periodical Articles

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