國家衛生研究院 NHRI:Item 3990099045/6747

NHRI > National Institute of Infectious Diseases and Vaccinology > Leung-Kei Siu > Periodical Articles > Item 3990099045/6747

Please use this identifier to cite or link to this item: http://ir.nhri.org.tw/handle/3990099045/6747

Title:	Molecular typing and resistance mechanisms of imipenem-non-susceptible Klebsiella pneumoniae in Taiwan: Results from the Taiwan Surveillance of Antibiotic Resistance (TSAR) study, 2002-2009
Authors:	Ma, L;Lu, PL;Siu, LK;Hsieh, MH
Contributors:	Division of Infectious Diseases
Abstract:	We investigated the molecular mechanisms and clonality of imipenem-non-susceptible Klebsiella pneumoniae isolates in a Taiwan national surveillance from 2002 to 2009. Genes for cabapenemases and plasmidic ampC-type and extended-spectrum beta-lactamases (EBSLs) were analysed by polymerase chain reaction. The major porin channels OmpK35 and OmpK36 were studied by sodium dodecyl sulphate-polyacrylamide gel electrophoresis. Molecular typing was performed with pulsed-field gel electrophoresis (PFGE) and multi-locus sequence typing (MLST). Our study revealed that all 29 isolates were ESBL producers. For K. pneumoniae in Taiwan from 2002 to 2009, most (84.6%, 11/13) imipenem-resistant (MIC > 2 mg/L) isolates carried bla(IMP-8). Isolates with an imipenem MIC of 2 mg/L produced ESBL with or without DHA-1 in combination with OmpK35/36 loss. PFGE analysis revealed that six small clusters of isolates were clonally related. The MLST grouping results were in concordance with the PFGE results. ST11, ST48 and ST101 were the predominant sequence types. Two novel ST types, ST1033 and ST1034, were found. The dominant clone in Taiwan, ST11, has been reported worldwide to be associated with various resistance mechanisms.
Date:	2013-01
Relation:	Journal of Medical Microbiology. 2013 Jan;63(Pt 1):101-107.
Link to:	http://dx.doi.org/10.1099/jmm.0.050492-0
JIF/Ranking 2023:	http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0022-2615&DestApp=IC2JCR
Cited Times(WOS):	https://www.webofscience.com/wos/woscc/full-record/WOS:000315607800012
Cited Times(Scopus):	http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84871123007
Appears in Collections:	[Leung-Kei Siu] Periodical Articles

Files in This Item:

File	Description	Size	Format
PUB23002067.pdf		2302Kb	Adobe PDF	501	View/Open

Loading...