國家衛生研究院 NHRI:Item 3990099045/6739

NHRI > National Institute of Infectious Diseases and Vaccinology > Pele Choi-Sing Chong > Periodical Articles > Item 3990099045/6739

Please use this identifier to cite or link to this item: http://ir.nhri.org.tw/handle/3990099045/6739

Title:	Immunological evaluation and comparison of different EV71 vaccine candidates
Authors:	Chou, AH;Liu, CC;Chang, JY;Lien, SP;Guo, MS;Tasi, HP;Hsiao, KN;Liu, SJ;Sia, C;Wu, SC;Lee, MS;Hsiao, CH;Wang, JR;Chow, YH;Chong, P
Contributors:	Division of Vaccine Research and Development
Abstract:	Enterovirus 71 (EV71) and coxsackievirus A16 (CVA16) are major causative agents of hand, foot, and mouth diseases (HFMDs), and EV71 is now recognized as an emerging neurotropic virus in Asia. Effective medications and/or prophylactic vaccines against HFMD are not available. The current results from mouse immunogenicity studies using in-house standardized RD cell virus neutralization assays indicate that (1) VP1 peptide (residues 211–225) formulated with Freund’s adjuvant (CFA/IFA) elicited low virus neutralizing antibody response (1/32 titer); (2) recombinant virus-like particles produced from baculovirus formulated with CFA/IFA could elicit good virus neutralization titer (1/160); (3) individual recombinant EV71 antigens (VP1, VP2, and VP3) formulated with CFA/IFA, only VP1 elicited antibody response with 1/128 virus neutralization titer; and (4) the formalin-inactivated EV71 formulated in alum elicited antibodies that cross-neutralized different EV71 genotypes (1/640), but failed to neutralize CVA16. In contrast, rabbits antisera could cross-neutralize strongly against different genotypes of EV71 but weakly against CVA16, with average titers 1/6400 and 1/32, respectively. The VP1 amino acid sequence dissimilarity between CVA16 and EV71 could partially explain why mouse antibodies failed to cross-neutralize CVA16. Therefore, the best formulation for producing cost-effective HFMD vaccine is a combination of formalin-inactivated EV71 and CAV16 virions.
Date:	2012-09
Relation:	Clinical and Developmental Immunology. 2012 Sep;2012:Article ID 831282.
Link to:	http://dx.doi.org/10.1155/2012/831282
Cited Times(WOS):	https://www.webofscience.com/wos/woscc/full-record/WOS:000309012400001
Cited Times(Scopus):	http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84867388819
Appears in Collections:	[Pele Choi-Sing Chong] Periodical Articles [Charles Dwo-Yuan Sia(2006-2014)] Periodical Articles [Joe Yen-Hung Chow] Periodical Articles [Shih-Jen Liu] Periodical Articles [Chia-Chyi Liu] Periodical Articles [Min-Shi Lee] Periodical Articles

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