國家衛生研究院 NHRI:Item 3990099045/6673
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    題名: An improved AhR reporter gene assay for analyzing dioxins in soil, sediment and fish
    作者: Chao, HR;Wang, YF;Wang, YN;Lin, DY;Gou, YY;Chen, CY;Chen, KC;Wu, WK;Chiang, BA;Huang, YT;Hsieh, LT;Yeh, KJC;Tsou, TC
    貢獻者: Division of Environmental Health and Occupational Medicine
    摘要: Our goal was to develop a fast-screening bioassay to determine dioxin levels in the environmental and biological samples from dioxin-contaminated areas. Our original dioxin-responsive-element (DRE)-driven luciferase bioassay (using Huh7-DRE-Luc cells) was modified by reducing the incubation temperature of the cell culture from 37 to 35°C and by adding phorbol-12-myristate-13-acetate, and the modified bioassay was used to examine samples from soil, sediment, and fish. The results of this bioassay were shown to be significantly related to those of the high-resolution gas chromatography/high-resolution mass spectrometry assay of dioxins. The correlative equation was: log (PCDD/Fs I-TEQs) = 1.19 × log (BEQs) − 1.15 with R2 = 0.95 (p < 0.001).
    日期: 2012-10
    關聯: Bulletin of Environmental Contamination and Toxicology. 2012 Oct;89(4):739-743.
    Link to: http://dx.doi.org/10.1007/s00128-012-0774-9
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0007-4861&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000308551500009
    Cited Times(Scopus): http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84867575767
    顯示於類別:[鄒粹軍] 期刊論文

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