國家衛生研究院 NHRI:Item 3990099045/6531
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    题名: A real time detection of the ovarian tumor associated antigen 1 (OVTA 1) in human serum by quartz crystal microbalance immobilized with anti-OVTA 1 polyclonal chicken IgY antibodies
    作者: Sadhasivam, S;Chen, JC;Savitha, S;Lin, FH;Yang, YY;Lee, CH
    贡献者: Division of Medical Engineering Research
    摘要: In this study, we developed a sensitive quartz crystal microbalance (QCM) sensor which employs the anti-ovarian tumor associated antigen 1 (OVTA 1) IgY polyclonal chicken antibodies on the crystal surface for the detection of OVTA 1 in human serum. The anti-OVTA 1 IgY antibodies were anchored on the thiol-activated sensor surface using 1-ethyl-3[3-dimethylaminopropyl] carbodiimide hydrochloride/N-hydroxysuccinimide (EDC-NHS) coupling. The responses for different concentrations of anti-OVTA 1 antibody were also studied and 40 μg/mL was favorable for homogenous antibody coverage. The QCM sensor was used to monitor both immobilization and the target binding affinity. The anti-OVTA 1 antibody based QCM has allowed the competent detection of OVTA 1 in a high linear range of 0.5-10 μg/mL. The total time needed to complete the detection was as short as 5-6 h. The regeneration studies demonstrated that the proposed sensor was reusable up to 9 cycles with a slight loss in binding affinity. The detection of OVTA 1 in human serum allows a potential exploitation of the anti-OVTA 1 polyclonal antibody based QCM immunoassay for the screening of OVTA 1 antigen.
    日期: 2012-10-01
    關聯: Materials Science and Engineering C. 2012 Oct 1;32(7):2073-2078.
    Link to: http://dx.doi.org/10.1016/j.msec.2012.05.043
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0928-4931&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000306937700048
    Cited Times(Scopus): http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84863306991
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