國家衛生研究院 NHRI:Item 3990099045/6465
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    题名: The effects of articular cartilage fragments and synovial membranes on chondrogenesis of bone marrow- derived mesenchymal stem cells
    作者: Chang, CH;Hu, JS;Chen, CC;Fang, HW;Hsu, YM
    贡献者: Division of Medical Engineering Research
    摘要: Purpose: There are many approaches have been developed to manage and treat articular cartilage injuries. For example, bone marrow stimulating techniques, mosaicplasty and autologous chondrocyte implantation. However, current clinical therapies still have some limitations regarding to the formation of ?brocartilage. On the other hand, tissue engineering of cartilage has been recognized as a new treatment for cartilage damage, but it still has some issues need to be veri?ed. In this study, we wanted to create an articular mimetic environment for better hyaline cartilage formation. Therefore, the components of the articular such as cartilage fragments and synovial membranes were added and the effects on chondrogenesis of bone marrow-derived mesenchymal stem cells (BM-MSCs) which were used for cartilage tissue engineering were investigated (schemed as ?gure 1). Methods: Cartilage fragments, synovial membranes and BM-MSCs were tested along or in different combinations (as table 1) for chondrogenesis. The components of different groups were wrapped by type I collagen gel and treated with induction medium. The matrix were analyzed at 0, 14, and 28 days after induction. Scanning electronic microscopy and histological staining by H&E and alcian blue were employed. Glycosaminoglycans (GAGs) content were measured and gene expression of Col I, Col II, Col X, SOX9, and aggrecan of matrix were analyzed as well. Results: Type II collagen gene expression of groups containing cartilage fragments were increased. When BM-MSCs were co-cultured with cartilage fragments and synovial membranes, histological and GAGs analysis showed that GAGs secretion from BM-MSCs was increased and the proliferation of BM-MSCs which closed to cartilage fragments was promoted. Finally, aggrecan and type II collagen expression of BM-MSCs were also increased while chondrocyte hypertrophy was inhibited after incubation for 2 weeks. Conclusions: We demonstrated that the articular cartilage fragments and synovial membranes in combination could promote chondrogenic differentiation of BM-MSCs. We hope these preliminary results could help develop an optimal condition for hyaline cartilage repair and be able to be applied along with bone marrow stimulating techniques in clinical treatment in the future.
    日期: 2012-04
    關聯: Osteoarthritis and Cartilage. 2012 Apr;20(Suppl. 1):S274.
    Link to: http://dx.doi.org/10.1016/j.joca.2012.02.465
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=1063-4584&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000303223300570
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