國家衛生研究院 NHRI:Item 3990099045/6405

NHRI > Institute of Molecular and Genomic Medicine > Gan-Guang Liou(2006-2014) > Periodical Articles > Item 3990099045/6405

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Title:	The self-interaction of native TDP-43 C terminus inhibits its degradation and contributes to early proteinopathies
Authors:	Wang, IF;Chang, HY;Hou, SC;Liou, GG;Way, TD;Shen, CKJ
Contributors:	Institute of Molecular and Genomic Medicine
Abstract:	The degraded, misfolded C terminus of TAR DNA-binding protein-43 is associated with a wide spectrum of neurodegenerative diseases, particularly frontotemporal lobar degeneration with ubiquitin-positive inclusions and amyotrophic lateral sclerosis. However, the precise mechanism of pathological cleavage of the TAR DNA-binding protein-43 remains unknown. Here we show that the TAR DNA-binding protein-43 C-terminal protein physically interacts with itself or with the cellular-folded yeast prion domain of Sup35 forming dynamic aggregates. This prion-like nature governs known cellular functions of the TAR DNA-binding protein-43, including subcellular localisation and exon skipping of the cystic fibrosis transmembrane conductance regulator. Significantly, mutants with a failure to engage in prion-like interactions are processed into an similar to 24-kDa C-terminal fragment of the TAR DNA-binding protein-43. The estimated cleavage site of degraded TAR DNA-binding protein-43 fragments corresponds to the pathological cleavage site identified in patients with the TAR DNA-binding protein-43 proteinopathies. Consistently, epigallocatechin gallate constrains prion-like interactions, attenuating pathological-like degradation. Thus, the native folding of TAR DNA-binding protein-43 C terminus acts as a guardian of pathogenesis, which is directly associated with loss-of-function.
Date:	2012-04
Relation:	Nature Communications. 2012 Apr;3:Article number 766.
Link to:	http://dx.doi.org/10.1038/ncomms1766
JIF/Ranking 2023:	http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=2041-1723&DestApp=IC2JCR
Cited Times(WOS):	https://www.webofscience.com/wos/woscc/full-record/WOS:000303455200003
Cited Times(Scopus):	http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84860271370
Appears in Collections:	[Gan-Guang Liou(2006-2014)] Periodical Articles

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