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http://ir.nhri.org.tw/handle/3990099045/6358
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| Title: | Distribution and phenotypic and genotypic detection of a metallo-Beta-lactamase, CphA, among bacteraemic Aeromonas isolates |
| Other Titles: | Distribution and phenotypic and genotypic detection of a metallo-β-lactamase, CphA, among bacteraemic Aeromonas isolates |
| Authors: | Wu, CJ;Chen, PL;Wu, JJ;Yan, JJ;Lee, CC;Lee, HC;Lee, NY;Chang, CM;Lin, YT;Chiu, YC;Ko, WC |
| Contributors: | Division of Infectious Diseases |
| Abstract: | The objectives of the study were to investigate the distribution of cphA-related genes (cphA)encoding a CphA metallo-b-lactamase (MBL) among 51 consecutive Aeromonas blood isolatesand to compare different phenotypic methods for detecting CphA. The presence of cphA wasdetected by PCR. Four phenotypic methods, the imipenem-EDTA combined disc test, imipenem-EDTA MBL Etest, agar dilution test and modified Hodge test (MHT), were used to detectimipenem susceptibility and MBL production. The results showed that 35 (69 %) blood isolateshad cphA. All (100 %) of 16 Aeromonas aquariorum isolates and 12 Aeromonas veronii isolates,and 4 (80 %) of 5 Aeromonas hydrophila isolates, carried cphA, but none of 15 Aeromonascaviae isolates did. With the standard inocula, irrespective of the presence or absence of cphA, allbut one (50, 98 %) isolates were susceptible to imipenem tested by disc diffusion, Etest and agardilution (104c.f.u. spot inocula), and did not exhibit MBL production by the imipenem-EDTAcombined disc test and MBL Etest. By the agar dilution test using large inocula (107c.f.u.), 34(97 %) of 35 cphA+isolates had imipenem MICs of ¢ 16 mg ml”1, higher than the susceptiblebreakpoint (4 mg ml”1), and demonstrated positive results for the MHT, while one cphA+and all17 cphA”isolates had imipenem MICs of ¡ 4 mg ml”1. In conclusion, the distribution of cphAamong aeromonads is species-specific, found in A. aquariorum, A. veronii and A. hydrophila, andthe MHT may be a phenotypic screening test for CphA production. |
| Date: | 2012-05 |
| Relation: | Journal of Medical Microbiology. 2012 May;61(5):712-719. |
| Link to: | http://dx.doi.org/10.1099/jmm.0.038323-0 |
| JIF/Ranking 2023: | http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0022-2615&DestApp=IC2JCR |
| Cited Times(WOS): | https://www.webofscience.com/wos/woscc/full-record/WOS:000304217700015 |
| Cited Times(Scopus): | http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84859869541 |
| Appears in Collections: | [吳綺容] 期刊論文
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| SCP84859869541.pdf | | 243Kb | Adobe PDF | 359 | View/Open |
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