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http://ir.nhri.org.tw/handle/3990099045/6315
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| Title: | Ethanol extracts of fruiting bodies of antrodia cinnamomea suppress CL1-5 human lung adenocarcinoma cells migration by inhibiting matrix metalloproteinase-2/9 through ERK, JNK, p38, and PI3K/Akt signaling pathways |
| Authors: | Chen, YY;Liu, FC;Chou, PY;Chien, YC;Chang, WSW;Huang, GJ;Wu, CH;Sheu, MJ |
| Contributors: | National Institute of Cancer Research |
| Abstract: | Cancer metastasis is a primary cause of cancer death. Antrodia cinnamomea (A. cinnamomea), a medicinal mushroom in Taiwan, has shown antioxidant and anticancer activities. In this study, we first observed that ethanol extract of fruiting bodies of A. cinnamomea (EEAC) exerted a concentration-dependent inhibitory effect on migration and motility of the highly metastatic CL1-5 cells in the absence of cytotoxicity. The results of a gelatin zymography assay showed that A. cinnamomea suppressed the activities of matrix metalloproteinase-(MMP-) 2 and MMP-9 in a concentration-dependent manner. Western blot results demonstrated that treatment with A. cinnamomea decreased the expression of MMP-9 and MMP-2; while the expression of the endogenous inhibitors of these proteins, that is, tissue inhibitors of MMP(TIMP-1 and TIMP-2) increased. Further investigation revealed that A. cinnamomea suppressed the phosphorylation of ERK1/2, p38, and JNK1/2. A. cinnamomea also suppressed the expressions of PI3K and phosphorylation of Akt. Furthermore, treatment of CL1-5 cells with inhibitors specific for PI3K (LY 294002), ERK1/2 (PD98059), JNK (SP600125), and p38 MAPK (SB203580) decreased the expression of MMP-2 and MMP-9. This is the first paper confirming the antimigration activity of this potentially beneficial mushroom against human lung adenocarcinoma CL1-5 cancer cells. |
| Date: | 2012-03 |
| Relation: | Evidence-based Complementary and Alternative Medicine. 2012 Mar;2012:Article ID 378415. |
| Link to: | http://dx.doi.org/10.1155/2012/378415 |
| Cited Times(WOS): | https://www.webofscience.com/wos/woscc/full-record/WOS:000301362600001 |
| Cited Times(Scopus): | http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84863269090 |
| Appears in Collections: | [張文祥] 期刊論文
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| ISI000301362600001.pdf | | 3642Kb | Adobe PDF | 815 | View/Open |
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