國家衛生研究院 NHRI:Item 3990099045/6295

NHRI > Institute of Cellular and Systems Medicine > Yasuhisa Fukui(2010-2016) > Periodical Articles > Item 3990099045/6295

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Title:	Membrane-induced alteration of the secondary structure in the SWAP-70 pleckstrin homology domain
Authors:	Tokuda, N;Kawai, K;Lee, YH;Ikegami, T;Yamaguchi, S;Yagisawa, H;Fukui, Y;Tuzi, S
Contributors:	Institute of Cellular and Systems Medicine
Abstract:	Differences in the conformation of the pleckstrin homology (PH) domain of switch-associated protein-70 (SWAP-70) in solution and at the lipid bilayer membrane surface were examined using CD, fluorescence and NMR spectroscopy. Intracellular relocalization of SWAP-70 from the cytoplasm to the plasma membrane and then to the nucleus is associated with its cellular functions. The PH domain of SWAP-70 contains a phosphoinositide-binding site and a nuclear localization signal, which localize SWAP-70 to the plasma membrane and nucleus, respectively. CD and fluorescence spectra showed that a significant conformational alteration involving formation of disordered structure occurs when the PH domain binds to d-myo-phosphatidylinositol 3,4,5-trisphosphate or d-myo-phosphatidylinositol 4,5-bisphosphate embedded in lipid bilayer vesicles. NMR spectra indicate that Ala and Trp residues located in the C-terminal alpha-helix of the PH domain undergo conformational alterations to form a disordered structure at the vesicle surface. These conformational alterations were not induced by association with inositol 1,3,4,5-tetrakisphosphate in solution or coexistence of phosphatidylcholine vesicles. Interaction with the plane of the lipid bilayer via association with the phosphoinositides is required for the unfolding of the C-terminal alpha-helix of the PH domain. The unwinding of the C-terminal alpha-helix could regulate the functions of SWAP-70 at the plasma membrane surface.
Date:	2012-04
Relation:	Journal of Biochemistry. 2012 Apr;151(4):391-401.
Link to:	http://dx.doi.org/10.1093/jb/mvr146
JIF/Ranking 2023:	http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0021-924X&DestApp=IC2JCR
Cited Times(WOS):	https://www.webofscience.com/wos/woscc/full-record/WOS:000302307100006
Cited Times(Scopus):	http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84859348330
Appears in Collections:	[Yasuhisa Fukui(2010-2016)] Periodical Articles

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