國家衛生研究院 NHRI:Item 3990099045/6172
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    题名: Generation of murine monoclonal antibodies which cross-neutralize human enterovirus genogroup B isolates
    作者: Chang, HW;Liu, CC;Lin, MH;Ho, HM;Yang, YT;Chow, YH;Chong, P;Sia, C
    贡献者: Vaccine Research and Development Center
    摘要: A live enterovirus 71 (EV71) isolate designated, EV71/E59, with genotype B4 produced in Vero cells and purified over a sucrose gradient was used as the immunogen to generate EV71-specific murine monoclonal antibodies. Four hybridoma clones derived from the fusion of splenocytes of EV71/E59-preimmunized BALB/c (H-2d) mice and the NS-1 myeloma cells that exhibit stable growth were selected for detailed characterization. The proof that the hybridomas produced are indeed true independent clones was based on the obervations that they expressed different complementarity-determining regions (CDRs) in their κ light chain genes. Purified ascitic fluids produced by the individual clones reacted against the viral capsid protein, VP1, in Western blot; and recognized distinct sites of a common epitope localized at the C-terminal half of VP1. Each of the monoclonal antibodies exhibited potent neutralizing activities against the immunizing virus strain, as well as two other isolates namely, N0781-TW-01, and N2838, of subgenogroups B4 and B5, respectively, that were found commonly in recent outbreaks in Taiwan. It was also observed the monoclonal antibodies acted cooperatively in neutralizing the EV71/E59 virus.
    日期: 2011-05
    關聯: Journal of Virological Methods. 2011 May;173(2):189-195.
    Link to: http://dx.doi.org/10.1016/j.jviromet.2011.02.003
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0166-0934&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000290836400005
    Cited Times(Scopus): http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=79954579605
    显示于类别:[謝鐸源(2006-2014)] 期刊論文
    [莊再成] 期刊論文
    [周彥宏] 期刊論文
    [劉家齊] 期刊論文

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