國家衛生研究院 NHRI:Item 3990099045/6095
English  |  正體中文  |  简体中文  |  全文筆數/總筆數 : 12145/12927 (94%)
造訪人次 : 862722      線上人數 : 782
RC Version 6.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
搜尋範圍 查詢小技巧：
  • 您可在西文檢索詞彙前後加上"雙引號"，以獲取較精準的檢索結果
  • 若欲以作者姓名搜尋，建議至進階搜尋限定作者欄位，可獲得較完整資料
    •  進階搜尋
    主頁 ‧ 登入 ‧ 上傳 ‧ 說明 ‧ 關於NHRI ‧ 管理 到手機版


    請使用永久網址來引用或連結此文件: http://ir.nhri.org.tw/handle/3990099045/6095


    題名: Identification of transforming hepatitis B virus S gene truncation mutants derived from freely replicative viruses in hepatocellular carcinomas
    作者: Huang, SFK;Chen, YT;Chiu, YT;Chang, Y;Yeh, CT
    貢獻者: Institute of Molecular and Genomic Medicine
    摘要: Background:. C-terminal truncated hepatitis B virus (HBV) surface protein has been reported to be associated with transcription activation activities. However, the studies on their roles in hepatocarcinogenesis remained limited. Material and Methods: From 1999 to 2002, we analyzed 300 HBV-related resected HCC specimens in Chang-Gung Memorial Hospital by immunohistochemistry and identified 28 hepatocellular carcinoma (HCC) tissues which were positive for hepatitis B core antigen (HBcAg) expression in the cancerous parts. Of them, 25 cases had frozen tumor tissues available for further studies. Freely replicative forms of HBV genomes were identified and isolated from the HBcAg-positive HCC tissues for sequence analysis. Plasmids encoding three HBV S truncation mutants were constructed to investigate their oncogenic potentials in NIH/3T3 cells. Tumorigenicity of these HBV S truncation mutants was evaluated by use of xenograft experiments in nude mice. The clinicopathological features of the patients carrying the mutants were compared with 25 sex- and age-matched HBcAg-negative HCC patients. Result: The 25 patients with HBcAg-positive HCC tissues included 20 males and 5 females. All patients had cirrhosis. Southern blot analysis for HBV-DNA in 16 tumors with sufficient tissue samples confirmed that active replicative forms of HBV genomes were present in these HCC tumors. Clinical data analysis suggested that most of the viable free HBV genomes were identified in early stage HCCs. Three deletions, and 3 nonsense mutations (sL95*, sW182* and sL216*) of the S gene were identified in 5 cancerous tissues and 3 non-cancerous issues, resulting in C-terminal truncations of the HBV surface proteins. Only the sW182* mutant was identified in 2 cancerous and 1 paired non-cancerous tissues, while other mutations were identified in one specimen only. Additional HBV-DNA sequence analysis in 56 HBV-related, HBcAg-negative HCC tumors was performed, which identified 7 tumors carrying the sW182* mutation, suggesting that the sW182* could be a frequently occurring HBV mutation in HCC. Functional studies of the 3 HBV S nonsense mutants revealed their transcription activation and transformation activities. Tumorigenicity analysis by xenograft experiments in nude mice showed that the sW182* mutant was the most potent oncogenic surface protein among the three mutants. Conclusion: The HBV S truncation mutants may play an important role in HBV-related hepatocarcinogenesis.
    日期: 2011-10
    關聯: Hepatology. 2011 Oct;54(Suppl 1):1293A.
    Link to: http://dx.doi.org/10.1002/hep.24666
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0270-9139&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000295578004441
    Cited Times(Scopus): http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=80054030408
    顯示於類別:[黃秀芬] 會議論文/會議摘要

    文件中的檔案:

    檔案 描述 大小格式瀏覽次數
    ISI000295578004441.pdf27KbAdobe PDF709檢視/開啟


    在NHRI中所有的資料項目都受到原著作權保護.

    TAIR相關文章

    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - 回饋