國家衛生研究院 NHRI:Item 3990099045/6065
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    題名: A single nucleotide in stem loop II of 5'-untranslated region contributes to virulence of Enterovirus 71 in mice
    作者: Yeh, MT;Wang, SW;Yu, CK;Lin, KH;Lei, HY;Su, IJ;Wang, JR
    貢獻者: Division of Infectious Diseases
    摘要: Background: Enterovirus 71 (EV71) has emerged as a neuroinvasive virus responsible for several large outbreaks in the Asia-Pacific region while virulence determinant remains unexplored. Principal Findings: In this report, we investigated increased virulence of unadapted EV71 clinical isolate 237 as compared with isolate 4643 in mice. A fragment 12 nucleotides in length in stem loop (SL) II of 237 59-untranslated region (UTR) visibly reduced survival time and rate in mice was identified by constructing a series of infectious clones harboring chimeric 59-UTR. In cells transfected with bicistronic plasmids, and replicon RNAs, the 12-nt fragment of isolate 237 enhanced translational activities and accelerated replication of subgenomic EV71. Finally, single nucleotide change from cytosine to uridine at base 158 in this short fragment of 59-UTR was proven to reduce viral translation and EV71 virulence in mice. Results collectively indicated a pivotal role of novel virulence determinant C158 on virus translation in vitro and EV71 virulence in vivo. Conclusions: These results presented the first reported virulence determinant in EV71 59-UTR and first position discovered from unadapted isolates.
    日期: 2011-11
    關聯: PLoS ONE. 2011 Nov;6(11):Article number e27082.
    Link to: http://dx.doi.org/10.1371/journal.pone.0027082
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=1932-6203&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000297150900050
    Cited Times(Scopus): http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=80055119062
    顯示於類別:[蘇益仁(2002-2015)] 期刊論文

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