國家衛生研究院 NHRI:Item 3990099045/6052

NHRI > Institute of Biotechnology and Pharmaceutical Research > Xin Chen(2002-2015) > Periodical Articles > Item 3990099045/6052

Please use this identifier to cite or link to this item: http://ir.nhri.org.tw/handle/3990099045/6052

Title:	Role of a propeller loop in the quaternary structure and enzymatic activity of prolyl dipeptidases DPP-IV and DPP9
Authors:	Tang, HK;Chen, KC;Liou, GG;Cheng, SC;Chien, CH;Tang, HY;Huang, LH;Chang, HP;Chou, CY;Chen, X
Contributors:	Institute of Biotechnology and Pharmaceutical Research;Institute of Molecular and Genomic Medicine
Abstract:	The dipeptidyl peptidase (DPP) family members, including DPP-IV, DPP8, DPP9 and others, cleave the peptide bond after the penultimate proline residue and are drug target rich. The dimerization of DPP-IV is required for its activity. A propeller loop located at the dimer interface is highly conserved within the family. Here we carried out site-directed mutagenesis on the loop of DPPIV and identified several residues important for dimer formation and enzymatic activity. Interestingly, the corresponding residues on DPP9 have a different impact whereby the mutations decrease activity without changing dimerization. Thus the propeller loop seems to play a varying role in different DPPs. STRUCTURED SUMMARY OF PROTEIN INTERACTIONS: DPP-IV and DPP-IVphysically interact by comigration in gel electrophoresis (View interaction: 1, 2, 3, 4) DPP9 and DPP9bind by circular dichroism (View interaction) DPP-IV and DPP-IVbind by circular dichroism (View interaction: 1, 2, 3, 4, 5) DPP-IV and DPP-IVbind by cosedimentation in solution (View interaction: 1, 2, 3, 4, 5) ADAbinds to DPP-IV by surface plasmon resonance (View interaction: 1, 2, 3, 4, 5, 6) DPP9 and DPP9bind by cosedimentation in solution (View interaction).
Date:	2011-11-04
Relation:	FEBS Letters. 2011 Nov 4;585(21):3409-3414.
Link to:	http://dx.doi.org/10.1016/j.febslet.2011.10.009
JIF/Ranking 2023:	http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0014-5793&DestApp=IC2JCR
Cited Times(WOS):	https://www.webofscience.com/wos/woscc/full-record/WOS:000296575400010
Cited Times(Scopus):	http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=80255122763
Appears in Collections:	[Xin Chen(2002-2015)] Periodical Articles [Gan-Guang Liou(2006-2014)] Periodical Articles

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