國家衛生研究院 NHRI:Item 3990099045/6041
English  |  正體中文  |  简体中文  |  Items with full text/Total items : 12145/12927 (94%)
Visitors : 856560      Online Users : 706
RC Version 6.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
Scope Tips:
  • please add "double quotation mark" for query phrases to get precise results
  • please goto advance search for comprehansive author search
    •  Adv. Search
    Home ‧ Login ‧ Upload ‧ Help ‧ About ‧ Administer Goto mobile version


    Please use this identifier to cite or link to this item: http://ir.nhri.org.tw/handle/3990099045/6041


    Title: Identification of the common regulators for hepatocellular carcinoma induced by hepatitis B virus X antigen in a mouse model
    Authors: Lu, JW;Yu, S;Yang, WY;Lin, YI;Li, CC;Tsai, TF;Chang, KW;Shieh, GS;Tsai, SF;Wang, HD;Yuh, CH
    Contributors: Institute of Molecular and Genomic Medicine
    Abstract: Hepatitis B virus X antigen plays an important role in the development of human hepatocellular carcinoma (HCC). The key regulators controlling the temporal downstream gene expression for HCC progression remains unknown. In this study, we took advantage of systems biology approach and analyzed the microarray data of the HBx transgenic mouse as a screening process to identify the differentially expressed genes, and applied the software Pathway Studio to identify potential pathways and regulators involved in HCC. Using sub-network enrichment analysis, we identified five common regulator genes: EDN1, BMP7, BMP4, SPIB and SRC. Up-regulation of the common regulators was validated in the other independent HBx transgenic mouse lines. Furthermore, we verified the correlation of their RNA expression levels by using the human HCC samples, and their protein levels by using the human liver disease tissue arrays. EDN1, BMP4 and BMP7 were up-regulated in cirrhosis, BMP4, BMP7, and SRC were further up-regulated in hepatocellular or cholangiocellular carcinoma samples. The trend of increasing expression of the common regulators correlates well with the progression of human liver cancer. Overexpression of the common regulators increases the cell viability, promotes migration and invasiveness, and enhances the colony formation ability in Hep3B cells. Our approach allows us to identify the critical genes in hepatocarcinogenesis in an HBx-induced mouse model. The validation of the gene expressions in the liver cancer of human patients and their cellular function assays suggests that the identified common regulators may serve as useful molecular targets for the early-stage diagnosis or therapy for HCC.
    Date: 2012-01
    Relation: Carcinogenesis. 2012 Jan;33(1):209-219.
    Link to: http://dx.doi.org/10.1093/carcin/bgr224
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0143-3334&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000298659200028
    Cited Times(Scopus): http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84855395049
    Appears in Collections:[Shih-Feng Tsai] Periodical Articles
    [Chiou-Hwa Yuh] Periodical Articles

    Files in This Item:

    File Description SizeFormat
    PUB22021908.pdf15976KbAdobe PDF752View/Open


    All items in NHRI are protected by copyright, with all rights reserved.

    Related Items in TAIR

    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - Feedback