國家衛生研究院 NHRI:Item 3990099045/5724

NHRI > National Institute of Infectious Diseases and Vaccinology > Ching-Liang Chu(2005-2007) > Periodical Articles > Item 3990099045/5724

Please use this identifier to cite or link to this item: http://ir.nhri.org.tw/handle/3990099045/5724

Title:	A novel adjuvant Ling Zhi-8 enhances the efficacy of DNA cancer vaccine by activating dendritic cells
Authors:	Lin, CC;Yu, YL;Shih, CC;Liu, KJ;Ou, KL;Hong, LZ;Chen, JDC;Chu, CL
Contributors:	Division of Vaccine Research and Development;National Institute of Cancer Research
Abstract:	DNA vaccine has been suggested to use in cancer therapy, but the efficacy remains to be improved. The immunostimulatory effect of a fungal immunomodulatory protein Ling Zhi-8 (LZ-8) isolated from Ganoderma lucidum has been reported. In this study, we tested the adjuvanticity of LZ-8 for HER-2/neu DNA vaccine against p185neu expressing tumor MBT-2 in mice. We found that recombinant LZ-8 stimulated mouse bone marrow-derived dendritic cells (DCs) via TLR4 and its stimulatory effect was not due to any microbe contaminant. In addition, LZ-8 enhanced the ability of DCs to induce antigen-specific T cell activation in vitro and in a subunit vaccine model in vivo. Surprisingly, LZ-8 cotreatment strongly improved the therapeutic effect of DNA vaccine against MBT-2 tumor in mice. This increase in antitumor activity was attributed to the enhancement of vaccine-induced Th1 and CTL responses. Consistent with the results from DCs, the promoting effect of LZ-8 on DNA vaccine was diminished when the MBT-2 tumor cells were grown in TLR4 mutant mice. Thus, we concluded that LZ-8 may be a promising adjuvant to enhance the efficacy of DNA vaccine by activating DCs via TLR4.
Date:	2011-07
Relation:	Cancer Immunology Immunotherapy. 2011 Jul;60(7):1019-1027.
Link to:	http://dx.doi.org/10.1007/s00262-011-1016-4
JIF/Ranking 2023:	http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0340-7004&DestApp=IC2JCR
Cited Times(WOS):	https://www.webofscience.com/wos/woscc/full-record/WOS:000291883500011
Cited Times(Scopus):	http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=85027957698
Appears in Collections:	[Ching-Liang Chu(2005-2007)] Periodical Articles [Ko-Jiunn Liu] Periodical Articles

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