國家衛生研究院 NHRI:Item 3990099045/5611
English  |  正體中文  |  简体中文  |  全文笔数/总笔数 : 12145/12927 (94%)
造访人次 : 860505      在线人数 : 672
RC Version 6.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
搜寻范围 查询小技巧：
  • 您可在西文检索词汇前后加上"双引号"，以获取较精准的检索结果
  • 若欲以作者姓名搜寻，建议至进阶搜寻限定作者字段，可获得较完整数据
    •  进阶搜寻
    主页 ‧ 登入 ‧ 上传 ‧ 说明 ‧ 关于NHRI ‧ 管理 到手机版


    jsp.display-item.identifier=請使用永久網址來引用或連結此文件: http://ir.nhri.org.tw/handle/3990099045/5611


    题名: Different culture medium formulations induce variant protein expression patterns of outer Membrane Porins in Klebsiella pneumoniae
    作者: Chen, JH;Lin, JC;Chang, JL;Tsai, YK;Siu, LK
    贡献者: Division of Infectious Diseases
    摘要: Outer membrane porin (OMP) expression has been shown to play an important role in antimicrobial resistance. In this study, we observed that OmpK35 of Klebsiella pneumoniae had varied expression profiles in different nutrient broths. the potential factors that could influence protein expression were assessed. K. pneumoniae (ATCC 13883) was cultured into two commercial available nutrient broths and also into solutions of the individual ingredients. To ensure that OmpK35 was detected, an OmpK35 deficient mutant was generated as control. When OmpK protein expression profiles were analyzed by SDS-PAGE, OmpK35 exhibited two different isoforms. Expression of an additional isoform-like OmpK35 protein was identified in one of the broths. No OmpK35 isoforms were observed when the individual ingredients of beef extract, casein or gelatin were used as culture medium. OmpK35 isoform expression could be repressed by adding more beef extract. In summary, OmpK can exhibit varied protein expression profiles when growing in different nutrient broths. The isoform-like protein expression of OmpK35 may lead to confusion in OmpK protein analysis.
    日期: 2011-02
    關聯: Journal of Chemotherapy. 2011 Feb;23(1):9-12.
    Link to: https://doi.org/10.1179/joc.2011.23.1.9
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=1120-009X&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000291705800002
    Cited Times(Scopus): http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=80051779220
    显示于类别:[蕭樑基] 期刊論文

    文件中的档案:

    没有与此文件相关的档案.



    在NHRI中所有的数据项都受到原著作权保护.

    TAIR相关文章

    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - 回馈