國家衛生研究院 NHRI:Item 3990099045/5433
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    题名: A single-monomer derived linear-like PEI-co-PEG for siRNA delivery and silencing
    作者: Tsai, LR;Chen, MH;Chien, CT;Chen, MK;Lin, FS;Lin, KMC;Hwu, YK;Yang, CS;Lin, SY
    贡献者: Center for Nanomedicine Research;Division of Medical Engineering Research
    摘要: Polyethylenimines (PEIs) are commonly used as a vehicle to deliver and protect siRNA, but the strong interaction still remains to be modulated for efficient siRNA release and silencing. Herein, a single-monomer derived linear-like PEI-co-PEG (LPEI-co-PEG, P2 ) was synthesized to substantially enhance the siRNA release, but not affect the efficiency of protection. The linear-like copolymer (P2 ) was only synthesized from a single-monomer by intensive synchrotron X-ray irradiation within 5?min, randomly producing both PEI and PEG segments. The counterpart vehicle, LPEI (P1 ), was also synthesized for comparison. We found that the P1 and P2 were able to prevent siRNA against enzymatic degradation. Most importantly, efficient siRNA release (52%) was only observed in the siRNA/P2 complexes and not in the siRNA/P1 complexes (<5%), suggesting that the PEG segment may modulate the interaction between siRNA and P2 segment. Specifically, P2 as well as P1 can emit photoluminescence; cancer cells exhibited a detectable photoluminescence after treatment with P1 and P2, indicative of their excellent transfection efficiency. Subsequently, the siGFP/P2 complexes knocked down GFP with excellent efficiency (75%) above the siGFP/P1 complexes (19%) and siGFP/Lipofectamine complexes (20%). Importantly, the siRNA with anti-VEGF function being associated with P2 have been demonstrated an excellent efficiency in the suppression of tumor growth.
    日期: 2011-05
    關聯: Biomaterials. 2011 May;32(14):3647-3653.
    Link to: http://dx.doi.org/10.1016/j.biomaterials.2011.01.059
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0142-9612&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000289020600012
    Cited Times(Scopus): http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=79952107509
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