國家衛生研究院 NHRI:Item 3990099045/5308
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    題名: Toward the detection of single cell releasing through high efficient chip-based nano fluidic systems
    其他題名: 5th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2010
    作者: Wu, RG;Wu, YS;Yang, CS;Tseng, FG
    貢獻者: Center for Nanomedicine Research
    摘要: By enhancing the speed, accuracy, and sensitivity, it opens up a new possibility for direct analysis of biologically relevant entities such as nucleic acids and proteins in single cell level. Nanofluidic systems with critical dimensions which were comparable to the molecular scales can provide the aforementioned performance, offering a novel basis for ultrasensitive and high-resolution bio-detections and medical diagnostics. Inspired by this concept, a nanofluidic system, integrated with a novel multi-nanocahnnel filter, an electrokinetic nano-preconcentrator, and a nanofluidic DAEKF detector for the detection of single cell releasing, is developed. A diluted solution of culture medium from PC-12 cells excited with excess nicotine concentration was detected with near single cell level amounts (c.a. 3000-10000 molecules) in this system after all processing in 20 min. Results showed that this multi-depth micro/nanofluidic chip exhibits superior sensitivity, efficiency, reliability, and reproducibility than those of conventional microchip electrophoresis /electrochromatography systems.
    日期: 2010-01-20
    關聯: 2010 IEEE 5th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2010. 2010 Jan 20-23:876-879.
    Link to: http://dx.doi.org/10.1109/NEMS.2010.5592133
    Cited Times(Scopus): http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=78649250589
    顯示於類別:[楊重熙] 會議論文/會議摘要

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