國家衛生研究院 NHRI:Item 3990099045/5293
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    题名: Isolating cells from blood using buoyancy activated cell sorting (BACS) with glass microbubbles
    作者: Hsu, CH;Chen, CC;Irimia, D;Toner, M
    贡献者: Division of Medical Engineering Research
    摘要: Obtaining pure cell subpopulations is often the first step for a wide range of applications. Here, we present a generic cell isolation strategy, the “buoyancy activated cell sorting (BACS)”, using glass microbubbles attached with target specific antibodies. The design of BACS was centered on the opposite movements of cells and microbubbles during mixing, ultimately improving the capture yield by increasing the number of interactions. Cells labeled with micro bubbles will spontaneously float and be isolated without the need for additional separation apparatuses. We demonstrated that more than 99% of CD4 positive cells in whole blood can be isolated in 15 minutes.
    日期: 2010
    關聯: International Conference on Miniaturized Systems for Chemistry and Life Sciences- TAS 2010. 2010:226-228.
    Link to: http://www.mendeley.com/research/isolating-cells-blood-using-buoyancy-activated-cell-sorting-bacs-glass-microbubbles/
    http://www.rsc.org/binaries/LOC/2010/PDFs/Papers/078_1000.pdf
    Cited Times(Scopus): http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84884328832
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