國家衛生研究院 NHRI:Item 3990099045/5201
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    题名: HNF-4alpha determines hepatic differentiation of human mesenchymal stem cells from bone marrow
    其它题名: HNF-4α determines hepatic differentiation of human mesenchymal stem cells from bone marrow
    作者: Chen, ML;Lee, KD;Huang, HC;Tsai, YL;Wu, YC;Kuo, TM;Hu, CP;Chang, C
    贡献者: Division of Molecular and Genomic Medicine
    摘要: AIM: To investigate the differentiation status and key factors to facilitate hepatic differentiation of human bone-marrow-derived mesenchymal stem cells (MSCs). METHODS: Human MSCs derived from bone marrow were induced into hepatocyte-like cells following a previously published protocol. The differentiation status of the hepatocyte-like cells was compared with various human hepatoma cell lines. Overexpression of hepatocyte nuclear factor (HNF)-4alpha was mediated by adenovirus infection of these hepatocyte-like cells. The expression of interesting genes was then examined by either reverse transcription-polymerase chain reaction (RT-PCR) or real-time RT-PCR methods. RESULTS: Our results demonstrated that the differentiation status of hepatocyte-like cells induced from human MSCs was relatively similar to poorly differentiated human hepatoma cell lines. Interestingly, the HNF-4 isoform in induced MSCs and poorly differentiated human hepatoma cell lines was identified as HNF-4gamma instead of HNF-4alpha. Overexpression of HNF-4alpha in induced MSCs significantly enhanced the expression level of hepatic-specific genes, liver-enriched transcription factors, and cytochrome P450 (P450) genes. CONCLUSION: Overexpression of HNF-4alpha improves the hepatic differentiation of human MSCs from bone marrow and is a simple way of providing better cell sources for clinical applications.
    日期: 2010-10-28
    關聯: World Journal of Gastroenterology. 2010 Oct 28;16(40):5092-5103.
    Link to: http://dx.doi.org/10.3748/wjg.v16.i40.5092
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=1007-9327&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000283675300013
    Cited Times(Scopus): http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=79952199658
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