國家衛生研究院 NHRI:Item 3990099045/5043

NHRI > National Institute of Environmental Health Sciences > Tsui-Chun Tsou > Periodical Articles > Item 3990099045/5043

Please use this identifier to cite or link to this item: http://ir.nhri.org.tw/handle/3990099045/5043

Title:	Zinc oxide particles induce inflammatory responses in vascular endothelial cells via NF-kappaB signaling
Other Titles:	Zinc oxide particles induce inflammatory responses in vascular endothelial cells via NF-κB signaling
Authors:	Tsou, TC;Yeh, SC;Tsai, FY;Lin, HJ;Cheng, TJ;Chao, HR;Tai, LA
Contributors:	Division of Environmental Health and Occupational Medicine;Center for Nanomedicine Research
Abstract:	This study investigated inflammatory effects of zinc oxide (ZnO) particles on vascular endothelial cells. The effects of 50 and 100-nm ZnO particles on human umbilical vein endothelial cells (HUVECs) were characterized by assaying cytotoxicity, cell proliferation, and glutathione levels. A marked drop in survival rate was observed when ZnO concentration was increased to 45 μg/ml. ZnO concentrations of ?3 μg/ml resulted in increased cell proliferation, while those of ?45 μg/ml caused dose-dependent increases in oxidized glutathione levels. Treatments with ZnO concentrations ?45 μg/ml were performed to determine the expression of intercellular adhesion molecule-1 (ICAM-1) protein, an indicator of vascular endothelium inflammation, revealing that ZnO particles induced a dose-dependent increase in ICAM-1 expression and marked increases in NF-κB reporter activity. Overexpression of IκBα completely inhibited ZnO-induced ICAM-1 expression, suggesting NF-κB plays a pivotal role in regulation of ZnO-induced inflammation in HUVECs. Additionally, TNF-α, a typical inflammatory cytokine, induced ICAM-1 expression in an NF-κB-dependent manner, and ZnO synergistically enhanced TNF-α-induced ICAM-1 expression. Both 50 and 100-nm ZnO particles agglomerated to similar size distributions. This study reveals an important role for ZnO in modulating inflammatory responses of vascular endothelial cells via NF-κB signaling, which could have important implications for treatments of vascular disease.
Date:	2010-11
Relation:	Journal of Hazardous Materials. 2010 Nov;183(1-3):182-188.
Link to:	http://dx.doi.org/10.1016/j.jhazmat.2010.07.010
JIF/Ranking 2023:	http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0304-3894&DestApp=IC2JCR
Cited Times(WOS):	https://www.webofscience.com/wos/woscc/full-record/WOS:000282607600023
Cited Times(Scopus):	http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=77956445167
Appears in Collections:	[Tsui-Chun Tsou] Periodical Articles [Others] Periodical Articles

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