This paper proposes a novel method employing gold-nanoparticle to enhance optic fiber Fabry-Perot (F-P) interferometry signal and applyed to immunoassay of rabbit IgG and anti-rabbit IgG. In the sensor preparation, cysteine molecules were immobilized on the sensor surface to allow attachment of gold-nanoparticles. Experiment results show that the spectrum shift can be improved by one order of magnitude as employing 40nm nanoparticles compared to the traditional detection ways by Febry-Perot interferometry. The minimum detectable concentration of 10<sup>7</sup> particles/μl was reached. Furthermore, the fiber sensor system was applied to the detection of immuno-reaction of rabbit-IgG and anti-rabbit IgG. The whole immuno sensing process including antibody binding and surface regeneration by elution took about 9 minutes, 3 times faster than the traditional way by micro dialysis. c2005 IEEE.
Date:
2005-07
Relation:
2005 5th IEEE Conference on Nanotechnology. 2005 Jul;2:117-120(Article number 1500665).
