國家衛生研究院 NHRI:Item 3990099045/4710

NHRI > National Institute of Environmental Health Sciences > Louis W. Chang(1999-2009) > Conference Papers/Meeting Abstract > Item 3990099045/4710

Please use this identifier to cite or link to this item: http://ir.nhri.org.tw/handle/3990099045/4710

Title:	Identification of genes and proteins associated with trans, trans-2,4-decadienal induced oxidative stress in human bronchial epithelial cells
Authors:	Lin, PP;Chang, LW;Liao, PC;Wu, HY;Tyan, YC;Tsai, HT
Contributors:	Division of Environmental Health and Occupational Medicine
Abstract:	Trans, trans-2,4-decadienal (tt-DDE), a specific type of dienaldehydes, is abundant in heated oils or cooking oil fumes. Ingestion of heated oils and exposure to cooking oil fumes has been suggested to have great health impact in variety of organs, including lung. We demonstrated that long-term treatment with tt-DDE increased cell proliferation and cytokines secretion via oxidative stress in human bronchial epithelial cells BEAS-2B. Furthermore, these effects were prevented by co-treatment with anti-oxidants N-acetylcysteine (NAC) and Vitamin C. Utilizing the proteomic techniques, the objective of this study was to identify protein biomarkers associated with tt-DDE-induced oxidative stress and cytotoxicity in human bronchial epithelial cells BEAS- 2B. Treatment with tt-DDE for 48 h dose-dependently reduced cell viability in human BEAS-2Bcells, but not in lung cancer cell lines. Co-treatment with antioxidant Nacetylcysteine tremendously reduced tt-DDE-induced in BEAS-2B cells. Protein extracts from DMSO or tt-DDE treated cells were separated on 2D gel and differentially expressed proteins were identified by mass spectrometry. A total of 30 proteins with known functions were identified
Date:	2006-09
Relation:	Toxicology Letters. 2006 Sep;164(Suppl.):S6.
Link to:	http://dx.doi.org/10.1016/j.toxlet.2006.06.016
JIF/Ranking 2023:	http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0378-4274&DestApp=IC2JCR
Cited Times(WOS):	https://www.webofscience.com/wos/woscc/full-record/WOS:000240407200009
Appears in Collections:	[Louis W. Chang(1999-2009)] Conference Papers/Meeting Abstract [Pinpin Lin] Conference Papers/Meeting Abstract

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