Disruptions in Schizophrenia 1 (DISC1) and Neuregulin 1 (NRG1) have been reported to be associated with schizophrenia in literature and inour previous association studies. The aim of this study is to search for risk polymorphisms of the two genes for schizophrenia, using the systematic mutation screening method. We have established standardised bioinformatic processes by using several tools to define the exonregions, promoter regions and highly conserved regions of the two genes. We have sequenced about 21 kb and 19 kb of the above regions of DISC1 and NRG1, respectively. Samples for direct sequencing are 100 schizophrenic patients and 50 normal controls. We have identified 93 genetic variations in DISC1, of which 63 have not been reported in NCBI dbSNP125, and 51 genetic variations in NRG1, of which 36 are novel. We will do genotyping work on 37 and 14 genetic variations inDISC1 and NRG1 respectively in large case-control samples.
Date:
2006-10
Relation:
American Journal of Medical Genetics Part B-Neuropsychiatric Genetics. 2006 Oct;141B(7):794.
