國家衛生研究院 NHRI:Item 3990099045/4672

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Title:	Adaptation of influenza H5N1 vaccine viruses in vero cells
Authors:	Tseng, YF;Weng, TC;Chen, YS;Hu, AYC;Chong, P;Lee, MS
Contributors:	Vaccine Research and Development Center
Abstract:	Current egg-based inﬂuenza vaccine production technology is labor-intensive and lack of ﬂexibility and its capacity would not be able to meet the demand during inﬂuenza pandemics. Therefore, vaccine production using mammalian cell technology is becoming viable and attractive. The current inﬂuenza H5N1 vaccine strain (NIBRG-14) could grow efﬁciently in chicken embroyonated eggs and MDCK cells ( ∼ 10 8 TCID50/ml) but not in Vero cells (10 5—6 TCID50/ml) which is the most common cell line for manufacturing human vaccines. After several passages and plaque puriﬁcations, two Vero-adapted high growth inﬂuenza H5N1 vaccine viruses (Vero-15 and Vero-16) have been selected and could reach high virus titer (10 8 TCID50/ml) in Vero cells in T ﬂasks. After tested with NIBRG-14 standard antiserum provided by the NIBSC, antigenicity of the Vero-15 and Vero-16 viruses remain similar to the NIBRG-14 virus. In addition, the Vero-15 and Vero-16 viruses do not have any nucleotide difference in HA and NA gene segments compared with the NIBRG-14. For process development, Vero cells were furture cultured on cytodex 1 microcarriers in spinner ﬂasks. Vero cells growed to 3 × 10 6 cells/ml with a seeding density of 4.4 × 10 5 cells/ml in 5 g/L microcarriers and peak virus titers reached 10 9 TCID50/ml. In conclusion, the Vero-15 and Vero-16 viruses are suitable for production of inﬂuenza H5N1 vaccines in Vero cells. Their other six internal gene segments could also be used to generate vaccine seed viruses of other inﬂuenza subtypes for production in Vero cells.
Date:	2008-12
Relation:	International Journal of Infectious Diseases. 2008 Dec;12(Suppl. 1):E253-E254.
Link to:	http://dx.doi.org/10.1016/j.ijid.2008.05.687
JIF/Ranking 2023:	http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=1201-9712&DestApp=IC2JCR
Cited Times(WOS):	https://www.webofscience.com/wos/woscc/full-record/WOS:000263287800633
Appears in Collections:	[Min-Shi Lee] Conference Papers/Meeting Abstract [Pele Choi-Sing Chong] Conference Papers/Meeting Abstract [Yung-Chih Hu] Conference Papers/Meeting Abstract

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