國家衛生研究院 NHRI:Item 3990099045/4662

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Please use this identifier to cite or link to this item: http://ir.nhri.org.tw/handle/3990099045/4662

Title:	Application of recombinant bamboo mosaic virus as vaccine to induce protective immunity in swine against foot-and-mouth disease virus
Authors:	Yang, CD;Liao, JT;Jong, MH;Liang, CM;Lin, YL;Lin, NS;Hsu, YH;Liang, SM
Contributors:	Division of Molecular and Genomic Medicine
Abstract:	Introduction: Plant viruses can be employed as versatile vectors for produc-tion of vaccines by expressing immunogenic epitopes on the surface of chi-meric viral particles. Although several viruses, including tobacco mosaicvirus, potato virus X and cowpea mosaic virus, have been developed as vectors, we aimed to develop a new viral vaccine delivery system, a bamboomosaic virus (BaMV), that carries larger transgene loads, and generates better immunity in the target animals with fewer adverse environmental effects.Methods: We engineered the BaMV as a vaccine vector expressing the anti-genic epitope(s) of the capsid protein VP1 of foot-and-mouth disease virus(FMDV). The recombinant BaMV plasmid (pBVP1) was constructed by replacing DNA encoding the 35 N-terminal amino acid residues of the BaMV coat protein with that encoding 37 amino acid residues (T128-N164)of FMDV VP1.Results: The pBVP1 infected host plants and generated a chimeric virionBVP1 expressing VP1 epitopes in its coat protein. Immunization of swine with BVP1 virions resulted in the production of anti-FMDV neutralizing antibodies and VP1-speciﬁc IFN-c. Furthermore, all BVP1-immunized swinewere protected against FMDV challenge. Conclusions: Chimeric BaMV virions that express partial sequence of FMDV VP1 effectively induced not only humoral and cell-mediated immune responses but also full protection against FMDV in target animals. This BaMV-based vector technology may be applied to other vaccines that require correct expression of antigens on chimeric viral particles.
Date:	2008-06
Relation:	FEBS Journal. 2008 Jun;275(Suppl. 1 ):362.
Link to:	http://dx.doi.org/10.1111/j.1742-4658.2008.06448.x
JIF/Ranking 2023:	http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=1742-464X&DestApp=IC2JCR
Cited Times(WOS):	https://www.webofscience.com/wos/woscc/full-record/WOS:000256633301260
Appears in Collections:	[Chi-Ming Liang(2006-2007)] Conference Papers/Meeting Abstract

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