The UV-irradiated host system has been used for identifying protein products of genes cloned in a phage vector. By starving the host cells for phosphate immediately before W-irradiation, we demonstrate that phosphate-regulated genes can be easily identified. By employing this new technique, we also provide evidence showing that the gpsA gene might be a new member of the phosphate starvation-inducible (psi) genes of E. coli.
Date:
1999-07
Relation:
Journal of Microbiological Methods. 1999 Jul;37(1):93-96.
