國家衛生研究院 NHRI:Item 3990099045/4434
English  |  正體中文  |  简体中文  |  Items with full text/Total items : 12145/12927 (94%)
Visitors : 857759      Online Users : 799
RC Version 6.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
Scope Tips:
  • please add "double quotation mark" for query phrases to get precise results
  • please goto advance search for comprehansive author search
    •  Adv. Search
    Home ‧ Login ‧ Upload ‧ Help ‧ About ‧ Administer Goto mobile version


    Please use this identifier to cite or link to this item: http://ir.nhri.org.tw/handle/3990099045/4434


    Title: Evaluation of the cytotoxicity of metallic dental materials on human oral epithelial cells and gigival fibroblasts
    Other Titles: 牙科用金屬材料對人類口腔細胞毒性之評估
    Authors: Lin, C;Tu, M;Chen, J;Chen, S
    Contributors: National Institute of Cancer Research
    Abstract: Purpose. We examinated the cytotoxicity of metallic dental material eluates on human oral epithelium cells and gingival fibroblasts. Methods. A total of five common metallic alloys, including Amalgam, Gold, Nickel, Titanium, and Platinum were tested. Five discs of each alloy were prepared, and each disc was 15 mm diameter and 2 mm thick. After being autoclaved, these alloy discs were incubated with 5 ml of culture medium at 37?C, with 5% CO<sub>2</sub> for 24 hours. The medium was then used to grow KB and HGF-1 cells. MTT assay examined the cytotoxicity of alloy eluates after 24-hour incubation. The effects of toxic alloy on cell cycle, necrosis, p53 and NF-kB activity, and DNA synthesis were further examined by flow cytometry, lactate dehydrogenase releasing assay, luciferase assay, and BrdU-incorporation, respectively. Results. Morphologic observation and the MTT test revealed that only the amalgam alloy was cytotoxic. Although flow cytometry showed no apoptotic cells after amalgam alloy treatment, high lactate dehydrogenase activity was found in culture medium. These results suggested that amalgam alloy induced breakdown of the cell membrane, a characteristic of necrosis. In addition, elevated p53 and NF-kB activity after amalgam alloy treatment supported that this alloy could cause inflammatory and stress responses. Finally, BrdU incorporation showed that amalgam alloy inhibited DNA synthesis. Conclusions. Based on the results of this experiment, casting metal alloys are safer than amalgam alloy.
    Date: 2006-12
    Relation: Mid-Taiwan Journal of Medicine. 2006 Dec;11(4):236-242.
    Link to: http://www.airitilibrary.com/searchdetail.aspx?DocIDs=10293507-200612-11-4-236-242-a
    Cited Times(Scopus): http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=33846324679
    Appears in Collections:[Jen-Yang Chen] Periodical Articles

    Files in This Item:

    File Description SizeFormat
    SCP33846324679.pdf4778KbAdobe PDF438View/Open


    All items in NHRI are protected by copyright, with all rights reserved.

    Related Items in TAIR

    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - Feedback