國家衛生研究院 NHRI:Item 3990099045/4278
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    Please use this identifier to cite or link to this item: http://ir.nhri.org.tw/handle/3990099045/4278


    Title: Regulation of FGF1 gene promoter through transcription factor RFX1
    Authors: Hsu, YC;Liao, WC;Kao, CY;Chiu, IM
    Contributors: Institute of Cellular and Systems Medicine
    Abstract: Fibroblast growth factor 1 (FGF1) has been suggested to have an important role in cell growth, proliferation, and neurogenesis. Human FGF1 gene 1B promoter (-540 to +31)-driven green fluorescence (F1BGFP) has been shown to monitor endogenous FGF1 expression. F1BGFP could also be used to isolate neural stem/progenitor cells (NSPCs) from embryonic, neonatal and adult mouse brains or to isolate glioblastoma stem cells (GBM-SCs) from human glioblastoma tissues. Here, we present evidence that transcription factor RFX1 could bind the 18-bp cis-elements (-484 to -467) of the F1B promoter, modulate F1BGFP expression, endogenous FGF1 expression, and further regulate the maintenance of GBM-SCs. These observations were substantiated by using yeast-one hybrid assay, electrophoretic mobility shift assay, chromatin immunoprecipitation assay, gain- and loss-of-function assays, and neurosphere assays. Overexpression of RFX1 was shown to down-regulate FGF-1B mRNA expression and neurosphere formation in human glioblastoma cells, whereas RNAi knockdown of RFX1 demonstrated the opposite effects. Our findings provide insight into FGF1 gene regulation and suggest that the roles of FGF1 and RFX1 in the maintenance of GBM-SCs. RFX1 may negatively regulate the self-renewal of GBM-SCs through modulating FGF-1B and FGF1 expression levels by binding the 18-bp cis-elements of F1B promoter.
    Date: 2010-04-30
    Relation: Journal of Biological Chemistry. 2010 Apr 30;285(18):13885-13895.
    Link to: http://dx.doi.org/10.1074/jbc.M109.081463
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=1083-351X&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000276987700063
    Cited Times(Scopus): http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=77951535377
    Appears in Collections:[Ing-Ming Chiu] Periodical Articles

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