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http://ir.nhri.org.tw/handle/3990099045/4276
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| Title: | The C-type lectin Clec12A present on mouse and human dendritic cells can serve as a target for antigen delivery and enhancement of antibody responses |
| Authors: | Lahoud, MH;Proietto, AI;Ahmet, F;Kitsoulis, S;Eidsmo, L;Wu, L;Sathe, P;Pietersz, S;Chang, HW;Walker, ID;Maraskovsky, E;Braley, H;Lew, AM;Wright, MD;Heath, WR;Shortman, K;Caminschi, I |
| Contributors: | Vaccine Research and Development Center |
| Abstract: | We have cloned the mouse and human C-type lectin Clec12A, expressed both, and produced mAb recognizing both. Mouse Clec12A is highly expressed on splenic CD8(+) dendritic cells (DC) and plasmacytoid DC. A proportion of CD8(-)DC also expresses lower levels of Clec12A, as do monocytes, macrophages, and B cells. Human CLEC12A, like the mouse counterpart, is expressed on blood monocytes and DC, including pDC and BDCA-3(+)DC, the proposed equivalent of mouse CD8(+)DC. To d etermine whether Ag targeted to Clec12A could induce immune responses, mice were injected with a rat mAb recognizing Clec12A, or a control rat mAb, then production of anti-rat Ig was measured. Anti-Clec12A mAb alone produced only moderate responses, but these were amplified by coinjecting only small amounts of LIPS as a DC activation agent. Furthermore, when OVA was conjugated to anti-Clec12A mAb, OVA-specific T cells were induced to proliferate. This Ag presentation to naive T cells was due to targeting conventional DC, because their ablation eliminated T cell activation. The potent Ab responses induced using microgram amounts of anti-Clec12A and minimal amounts of adjuvant demonstrate that this molecule can be used as an Ag-delivery target to enhance Ab responses to vaccines. The Journal of Immunology, 2009, 182: 7587-7594. |
| Date: | 2009-06-15 |
| Relation: | Journal of Immunology. 2009 Jun 15;182(12):7587-7594. |
| Link to: | http://dx.doi.org/10.4049/jimmunol.0900464 |
| JIF/Ranking 2023: | http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0022-1767&DestApp=IC2JCR |
| Cited Times(WOS): | https://www.webofscience.com/wos/woscc/full-record/WOS:000266833900029 |
| Cited Times(Scopus): | http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=67649184779 |
| Appears in Collections: | [其他] 期刊論文
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| ISI000266833900029.pdf | | 2225Kb | Adobe PDF | 938 | View/Open |
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