國家衛生研究院 NHRI:Item 3990099045/4234
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    题名: Phytoestrogen bavachin mediates anti-inflammation targeting IkappaB kinase-IkappaBalpha-NF-kappaB signaling pathway in chondrocytes in vitro
    其它题名: Phytoestrogen bavachin mediates anti-inflammation targeting IκB kinase-IκBα-NF-κB signaling pathway in chondrocytes in vitro
    作者: Cheng, CC;Chen, YH;Chang, WL;Yang, SP;Chang, DM;Lai, JH;Ho, LJ
    贡献者: Institute of Cellular and Systems Medicine
    摘要: The pro-inflammatory cytokine interleukin-1beta (IL-1beta) plays critical roles in pathogenesis of osteoarthritis. Although estrogen is protective for cartilage in osteoarthritis patients, it also potentially increases the risk of stroke and cancer. Phytoestrogens acting as natural estrogen receptor modulators may serve as alternatives. This study aimed to identify medicinal phytoestrogens that preserve anti-inflammatory property and may function as potential chondro-protective compounds. Both human chondrocytes and chondrocytic cell line CHON-002 were used for this study. Protein concentrations or expressions were measured by ELISA or Western blot, respectively. The DNA-binding activity and transcriptional activity of transcription factors were evaluated by electrophoretic mobility shift assay and dual-luciferase reporter assay, respectively. Cell migration was analyzed by chemotaxis assays. We found that among screened phytoestrogens, bavachin could potently decrease IL-1beta-induced nuclear factor-kappa B (NF-kappaB) but not activator protein-1 (AP-1) DNA-binding activity. Bavachin also inhibited IkappaBalpha degradation, increased nuclear translocation of p65 and p50 as well as decreased IkappaBalpha kinase (IKK) activity. Furthermore, bavachin inhibited IL-1beta-induced chemokine production that resulted in reduced migration of THP-1 monocytic cells. Our results suggest that through decreasing IL-1beta-induced activation of IKK-IkappaBalpha-NF-kappaB signaling pathway, bavachin potentially protects cartilage from inflammation-mediated damage in joints of osteoarthritis patients.
    日期: 2010-06-25
    關聯: European Journal of Pharmacology. 2010 Jun 25;636(1-3):181-188.
    Link to: http://dx.doi.org/10.1016/j.ejphar.2010.03.031
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0014-2999&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000278425200025
    Cited Times(Scopus): http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=77952645505
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