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http://ir.nhri.org.tw/handle/3990099045/4228
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| Title: | 4 beta-hydroxywithanolide E from physalis peruviana (golden berry) inhibits growth of human lung cancer cells through DNA damage, apoptosis and G(2)/M arrest |
| Other Titles: | 4β-Hydroxywithanolide E from Physalis peruviana (golden berry) inhibits growth of human lung cancer cells through DNA damage, apoptosis and G2/M arrest |
| Authors: | Yen, CY;Chiu, CC;Chang, FR;Chen, JYF;Hwang, CC;Hseu, YC;Yang, HL;Lee, AYL;Tsai, MT;Guo, ZL;Cheng, YS;Liu, YC;Lan, YH;Chang, YC;Ko, YC;Chang, HW;Wu, YC |
| Contributors: | Division of Environmental Health and Occupational Medicine |
| Abstract: | Background: The crude extract of the fruit bearing plant, Physalis peruviana (golden berry), demonstrated anti-hepatoma and anti-inflammatory activities. However, the cellular mechanism involved in this process is still unknown. Methods: Herein, we isolated the main pure compound, 4 beta-Hydroxywithanolide (4 beta HWE) derived from golden berries, and investigated its antiproliferative effect on a human lung cancer cell line (H1299) using survival, cell cycle, and apoptosis analyses. An alkaline comet-nuclear extract (NE) assay was used to evaluate the DNA damage due to the drug. Results: It was shown that DNA damage was significantly induced by 1, 5, and 10 mu g/mL 4 beta HWE for 2 h in a dose-dependent manner (p < 0.005). A trypan blue exclusion assay showed that the proliferation of cells was inhibited by 4 beta HWE in both dose- and time-dependent manners (p < 0.05 and 0.001 for 24 and 48 h, respectively). The half maximal inhibitory concentrations (IC50) of 4 beta HWE in H1299 cells for 24 and 48 h were 0.6 and 0.71 mu g/mL, respectively, suggesting it could be a potential therapeutic agent against lung cancer. In a flow cytometric analysis, 4 beta HWE produced cell cycle perturbation in the form of sub-G(1) accumulation and slight arrest at the G(2)/M phase with 1 mu g/mL for 12 and 24 h, respectively. Using flow cytometric and annexin V/propidium iodide immunofluorescence double-staining techniques, these phenomena were proven to be apoptosis and complete G(2)/M arrest for H1299 cells treated with 5 mu g/mL for 24 h. Conclusions: In this study, we demonstrated that golden berry-derived 4 beta HWE is a potential DNA-damaging and chemotherapeutic agent against lung cancer. |
| Date: | 2010-02 |
| Relation: | BMC Cancer. 2010 Feb;10(46):Article Number 46. |
| Link to: | http://dx.doi.org/10.1186/1471-2407-10-46 |
| JIF/Ranking 2023: | http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=1471-2407&DestApp=IC2JCR |
| Cited Times(WOS): | https://www.webofscience.com/wos/woscc/full-record/WOS:000275208700001 |
| Cited Times(Scopus): | http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=77649104750 |
| Appears in Collections: | [葛應欽(2003-2009)] 期刊論文
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| ISI000275208700001.pdf | | 914Kb | Adobe PDF | 824 | View/Open |
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