國家衛生研究院 NHRI:Item 3990099045/4109
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    題名: Differences in gene expression between sonoporation in tumor and in muscle
    作者: Tsai, KC;Liao, ZK;Yang, SJ;Lin, WL;Shieh, MJ;Hwang, LH;Chen, WS
    貢獻者: Division of Medical Engineering Research
    摘要: Background: Ultrasound (US) is a novel and effective tool for the local delivery of genes into target tissues. US can temporarily change the permeability of a cell membrane and thus enhance the delivery of naked DNA into cells. In the present study, the efficiencies of gene expression mediated by US delivery in orthotopic liver tumor, subcutaneous tumor and muscle tissue were evaluated by changing the contrast agent concentrations and US exposure durations. Methods: Plasmid DNA coding for luciferase, interleukin-12 or enhanced green fluorescence protein was mixed with SonoVueR and injected intratumorally or intramuscularly. The injection sites were then exposed to US (20% duty cycle and 0.4 W/cm2 intensity). Results: The results obtained showed that the optimal condition was 50% SonoVueR for tumors and 30% for muscle, with 10 min of US exposure. The expression levels of the transfected DNAs were in the order: muscle > subcutaneous tumor > orthotopic liver tumor. Conclusions: The present study indicates that muscle tissue is a good target site for producing large amounts of gene products for the purpose of gene therapy.
    日期: 2009-10
    關聯: Journal of Gene Medicine. 2009 Oct;11(10):933-940.
    Link to: http://dx.doi.org/10.1002/jgm.1376
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=1099-498X&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000271068800008
    Cited Times(Scopus): http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=72449131037
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