國家衛生研究院 NHRI:Item 3990099045/3980

NHRI > National Institute of Infectious Diseases and Vaccinology > Min-Shi Lee > Periodical Articles > Item 3990099045/3980

Please use this identifier to cite or link to this item: http://ir.nhri.org.tw/handle/3990099045/3980

Title:	Development of a high-throughput assay for measuring serum neutralizing antibody against enterovirus 71
Authors:	Huang, ML;Chiang, PS;Luo, ST;Liou, GY;Lee, MS
Contributors:	Vaccine Research and Development Center
Abstract:	Enterovirus 71 (EV71) is the main etiologic agent of hand, foot, and mouth disease (HFMD) and causes frequently severe neurological complications and mortality in young children. The serum neutralizing antibody response is the major indicator of EV71 infection and protective immunity. The current serum neutralization test based on inhibition of cytopathic effect (Nt-CPE) requires manual microscopic examination, which is time-consuming and labor-intensive. In this study, a high-throughput neutralization assay which employs enzyme immunoassay for detecting growth of EV71 in Rhabdomyosarcoma (RD) cells and measuring serum neutralizing antibody (Nt-EIA) against EV71 was developed. RD cells infected with 100 TCID50 of EV71 for 36-42 h had the best performance and were selected for Nt-EIA. One hundred and twenty human sera (59 negative sera, 61 positive sera) were measured for EV71 neutralization antibody titers by Nt-CPE and Nt-EIA. Neutralization antibody titers against EV71 determined by Nt-EIA had a high sensitivity (100%), specificity (94.9%) and agreement (97.5%) by a qualitative comparison with Nt-CPE. In the quantitative comparison, the correlation coefficient between Nt-EIA and Nt-CPE was 0.91 after log transformation. Overall, the Nt-EIA is a suitable alternative assay for the quantitation of EV71 neutralizing antibody to EV71.
Date:	2010-04
Relation:	Journal of Virological Methods. 2010 Apr;165(1):42-45.
Link to:	http://dx.doi.org/10.1016/j.jviromet.2009.12.015
JIF/Ranking 2023:	http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0166-0934&DestApp=IC2JCR
Cited Times(WOS):	https://www.webofscience.com/wos/woscc/full-record/WOS:000276651800007
Cited Times(Scopus):	http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=77649339138
Appears in Collections:	[Min-Shi Lee] Periodical Articles

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