國家衛生研究院 NHRI:Item 3990099045/3953

NHRI > National Institute of Environmental Health Sciences > Ying-Chin Ko(2003-2009) > Periodical Articles > Item 3990099045/3953

Please use this identifier to cite or link to this item: http://ir.nhri.org.tw/handle/3990099045/3953

Title:	6-shogaol, an active constituent of dietary ginger, induces autophagy by inhibiting the AKT/mTOR pathway in human non-small cell lung cancer A549 cells
Authors:	Hung, JYU;Hsu, YAL;Li, CT;Ko, YC;Ni, WC;Huang, MS;Kuo, PL
Contributors:	Division of Environmental Health and Occupational Medicine
Abstract:	This study is the first study to investigate the anticancer effect of 6-shogaol in human non-small cell lung cancer A549 cells. 6-Shogaol inhibited cell proliferation by Inducing autophagic cell death, but not, predominantly, apoptosis. Pretreatment of cells with 3-methyladenine (3-MA), an autophagy inhibitor, suppressed 6-shogaol mediated antiproliferation activity, suggesting that induction of autophagy by 6-shogaol is conducive to cell death. We also found that 6-shogaol inhibited survival signaling through the AKT/mTOR signaling pathway by blocking the activation of AKT and downstream targets, including the mammalian target of rapamycin (mTOR), forkhead transcription factors (FKHR) and glycogen synthase kinase-3β (GSK-3β). Phosphorylation of both of mTOR's downstream targets, p70 ribosomal protein S6 kinase (p70S6 kinase) and 4E-BP1, was also diminished. Overexpression of AKT by AKT cDNA transfection decreased 6-shogaol mediated autophagic cell death, supporting inhibition of AKT beneficial to autophagy. Moreover, reduction of AKT expression by siRNA potentiated 6-shogaol's effect, also supporting inhibition of AKT beneficial to autophagy. Taken together, these findings suggest that 6-shogaol may be a promising chemopreventive agent against human non-small cell lung cancer.
Date:	2009-10-28
Relation:	Journal of Agricultural and Food Chemistry. 2009 Oct 28;57(20):9809-9816.
Link to:	http://dx.doi.org/10.1021/jf902315e
JIF/Ranking 2023:	http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0021-8561&DestApp=IC2JCR
Cited Times(WOS):	https://www.webofscience.com/wos/woscc/full-record/WOS:000270858200065
Cited Times(Scopus):	http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=70350317873
Appears in Collections:	[Ying-Chin Ko(2003-2009)] Periodical Articles

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