國家衛生研究院 NHRI:Item 3990099045/3938
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    題名: Identification of HLA-A11-restricted CTL epitopes derived from HPV type 18 using DNA immunization
    作者: Chen, HW;Leng, CH;Liu, HY;Cheng, WF;Chang, YW;Wu, PY;Lien, SP;Huang, TY;Chiang, SK;Lin, MH;Tao, MH;Chong, P;Liu, SJ
    貢獻者: Vaccine Research and Development Center
    摘要: Identification of the cytotoxic T lymphocyte (CTL) epitopes of tumor antigens is important for effective immunotherapy. We report that a combination of epitope prediction, enzyme-linked immunosorbent assay (ELISA)-based epitope-HLA complex formation, and DNA immunization methods can improve the efficiency and accuracy of CTL epitope studies. In this study, two HLA-A11-restricted epitopes derived from human papillomavirus (HPV)18 E6 oncoprotein were identified. HLA-A11-transgenic mice immunized with these epitopes could specifically induce interferon-a (IFNa) production, cytotoxicity and peptide/HLA-A11 tetramer binding in CD8+ T-cells. To study intracellular processing of CTL epitopes, we constructed a DNA plasmid containing an endoplasmic reticulum (ER) targeting sequence as well as the HPV18 E6 and E7 genes (pEK/HPV18E6E7). CTL responses against peptide-pulsed T2/A11 cells could be detected after immunizing HLA-A11-transgenic mice with pEK/HPV18E6E7. Furthermore, the identified peptides could stimulate T-cells to secrete IFNa from HPV18-infected patients. Our results demonstrate that the antigenic E6 peptides derived from HPV18 are potential candidates for the treatment of HPV 18-associated tumors in HLA-A11+ populations.
    日期: 2009-11-01
    關聯: Cancer Biology and Therapy. 2009 Nov 1;8(21):2025-2032.
    Link to: http://www.landesbioscience.com/journals/cbt/article/9732/
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=1538-4047&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000272796200010
    Cited Times(Scopus): http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=73549088284
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