國家衛生研究院 NHRI:Item 3990099045/3911

NHRI > Institute of Population Health Sciences > MARK LAWRENCE WAHLQVIST(2008-2012) > Periodical Articles > Item 3990099045/3911

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Title:	Docosahexaenoic acid decreases plasma homocysteine via regulating enzyme activity and mRNA expression involved in methionine metabolism
Authors:	Huang, T;Wahlqvist, ML;Li, D
Contributors:	Division of Health Policy Research and Development
Abstract:	Objective: The objective of this study was to investigate the regulatory effect of fish oil rich in ω-3 polyunsaturated fatty acids (PUFAs) on critical enzyme activity and mRNA expression involved in homocysteine (Hcy) metabolism. Methods: Thirty-six male Sprague-Dawley rats aged 3 wk, weighing 120 ± 10 g, were randomly divided into three groups: the olive oil (OO) group, the tuna oil (TO) group, and the salmon oil (SO) group. The oil was orally administered every day using a stomach tube. Eight weeks later, plasma Hcy, phospholipids, ω-3 PUFAs, enzyme activity, and mRNA expression in tissues were determined. Results: Compared with the control group, phospholipids, total ω-3 PUFAs, and ω-3/ω-6 PUFAs in the liver and lung were significantly elevated in the TO and SO groups; 22:6ω-3 in the liver and lung was significantly increased in the TO group; and 20:5ω-3 in the two tissues was significantly elevated in the SO group. The level of plasma Hcy was significantly decreased with TO; methionine adenosyl transferase (MAT) activity was significantly increased and MAT mRNA expression was significantly upregulated with TO; cystathionine-γ-lyase mRNA expression in TO was significantly upregulated; however, cystathionine β-synthase and S-adenosylhomocysteine hydrolases were not significantly changed when compared with control. Conclusion: TO rich in 22:6ω-3 decreases the concentration of Hcy despite increasing MAT activity and upregulating MAT mRNA expression through compensatory cystathionine-γ-lyase mRNA expression, both of which are involved in Hcy metabolism.
Date:	2010-01
Relation:	Nutrition. 2010 Jan;26(1):112-119.
Link to:	http://dx.doi.org/10.1016/j.nut.2009.05.015
JIF/Ranking 2023:	http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0899-9007&DestApp=IC2JCR
Cited Times(WOS):	https://www.webofscience.com/wos/woscc/full-record/WOS:000276331800013
Cited Times(Scopus):	http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=71949093334
Appears in Collections:	[MARK LAWRENCE WAHLQVIST(2008-2012)] Periodical Articles

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