國家衛生研究院 NHRI:Item 3990099045/3907
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    題名: A pre-S gene chip to detect pre-S deletions in hepatitis B virus large surface antigen as a predictive marker for hepatoma risk in chronic hepatitis B virus carriers
    作者: Shen, FC;Su, IJ;Wu, HC;Hsieh, YH;Yao, WJ;Young, KC;Chang, TC;Hsieh, HC;Tsai, HN;Huang, W
    貢獻者: Division of Infectious Diseases
    摘要: Background. Chronic hepatitis B virus (HBV) infection is an important cause of hepatocellular carcinoma (HCC) worldwide. The pre-S1and -S 2mutant large HBV surface antigen (LHBS), in which the pre-S 1and -S2regions of the LHBS gene are partially deleted, are highly associated with HBV-related HCC. Methods. The pre-S region of the LHBS gene in two hundred and one HBV-positive serum samples was PCR-amplified and sequenced. A pre-S oligonucleotide gene chip was developed to efficiently detect pre-S deletions in chronic HBV carriers. Twenty serum samples from chronic HBV carriers were analyzed using the chip. Results. The pre-S deletion rates were relatively low (7%) in the sera of patients with acute HBV infection. They gradually increased in periods of persistent HBV infection: pre-S mutation rates were 37% in chronic HBV carriers, and as high as 60% in HCC patients. The Pre-S Gene Chip offers a highly sensitive and specific method for pre-S deletion detection and is less expensive and more efficient (turnaround time 3 days) than DNA sequencing analysis. Conclusion. The pre-S1/2mutants may emerge during the long-term persistence of the HBV genome in carriers and facilitate HCC development. Combined detection of pre-S mutations, other markers of HBV replication, and viral titers, offers a reliable predictive method for HCC risks in chronic HBV carriers.
    日期: 2009-09-15
    關聯: Journal of Biomedical Science. 2009 Sep 15;16:Article number 84.
    Link to: http://dx.doi.org/10.1186/1423-0127-16-84
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=1021-7770&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000270925300001
    Cited Times(Scopus): http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=70349687648
    顯示於類別:[蘇益仁(2002-2015)] 期刊論文

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