國家衛生研究院 NHRI:Item 3990099045/3856

NHRI > Institute of Biomedical Engineering and Nanomedicine > Kurt Ming-Chao Lin > Periodical Articles > Item 3990099045/3856

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Title:	The use of injectable spherically symmetric cell aggregates self-assembled in a thermo-responsive hydrogel for enhanced cell transplantation
Authors:	Lee, WY;Chang, YH;Yeh, YC;Chen, CH;Lin, KM;Huang, CC;Chang, Y;Sung, HW
Contributors:	Division of Medical Engineering Research
Abstract:	Typical cell transplantation techniques involve the administration of dissociated cells directly injected into muscular tissues; however, retention of the transplanted cells at the sites of the cell graft is frequently limited. An approach, using spherically symmetric aggregates of cells with a relatively uniform size self-assembled in a thermo-responsive methylcellulose hydrogel system, is reported in the study. The obtained cell aggregates preserved their endogenous extracellular matrices (ECM) and intercellular junctions because no proteolytic enzyme was used when harvesting the cell aggregates. Most of the cells within aggregates (with a radius of approximately 100 μm) were viable as indicated by the live/dead staining assay. After injection through a needle, the cell aggregates remained intact and the cells retained their activity upon transferring to another growth surface. The cell aggregates obtained under sterile conditions were transplanted into the skeletal muscle of rats via local injection. The dissociated cells were used as a control. It was found that the cell aggregates can provide an adequate physical size to entrap into the muscular interstices and offer a favorable ECM environment to enhance retention of the transplanted cells at the sites of the cell graft. These results indicated that the spherically symmetric cell aggregates developed in the study may serve as a cell delivery vehicle for therapeutic applications.
Date:	2009-10
Relation:	Biomaterials. 2009 Oct;30(29):5505-5513.
Link to:	http://dx.doi.org/10.1016/j.biomaterials.2009.07.006
JIF/Ranking 2023:	http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0142-9612&DestApp=IC2JCR
Cited Times(WOS):	https://www.webofscience.com/wos/woscc/full-record/WOS:000270115200028
Cited Times(Scopus):	http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=68549118666
Appears in Collections:	[Kurt Ming-Chao Lin] Periodical Articles

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