國家衛生研究院 NHRI:Item 3990099045/3614
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    题名: Generating and characterizing monoclonal and polyclonal antibodies against avian H5N1 hemagglutinin protein
    作者: Wang, SF;Chen, KH;Thitithanyanont, A;Yao, L;Lee, YM;Chan, YJ;Liu, SJ;Chong, P;Liu, WT;Huang, JC;Chen, YMA
    贡献者: Vaccine Research and Development Center
    摘要: Accurate and timely diagnoses are central to H5N1 infection control. Here we describe the cloning and expression of the HA1 protein of the A/Vietnam/1203/04 strain in a bacterial system to generate mono-/polyclonal antibodies. All of the eight generated monoclonal antibodies recognized the same linear epitope on the top globular region of the HA structure-a highly conserved epitope among all circulating H5N1 clades identified by amino acid alignment. Results from immunofluorescence staining and Western blotting indicate that all monoclonal antibodies interacted with a denatured form of HA proteins, while the resultant polyclonal antibodies recognized both denatured and native HA proteins on H5N1 reverse-genetics (RG) viruses. Results from flow cytometry and microneutralization assays indicate that the polyclonal antibodies blocked viral binding and neutralized H5N1-RG viruses. Our results may prove useful to establishing future H5N1 mono-and polyclonal antibodies, and perhaps contribute to the development of an alternative H5N1 vaccine.
    日期: 2009-05-15
    關聯: Biochemical and Biophysical Research Communications. 2009 May 15;382(4):691-696.
    Link to: http://dx.doi.org/10.1016/j.bbrc.2009.03.119
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0006-291X&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000265989200010
    Cited Times(Scopus): http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=64749097242
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