國家衛生研究院 NHRI:Item 3990099045/3613

NHRI > National Institute of Infectious Diseases and Vaccinology > Pele Choi-Sing Chong > Periodical Articles > Item 3990099045/3613

Please use this identifier to cite or link to this item: http://ir.nhri.org.tw/handle/3990099045/3613

Title:	Development of multi-phase emulsions based on bioresorbable polymers and oily adjuvant
Authors:	Huang, MH;Huang, CY;Lien, SP;Siao, SY;Chou, AH;Chen, HW;Liu, SJ;Leng, CH;Chong, P
Contributors:	Vaccine Research and Development Center
Abstract:	Purpose: To enhance the water affinity of W/O emulsion-adjuvanted vaccines, we used three bioresorbable polymers named PEG-b-PLA, PEG-b-PCL, and PEG-b-PLACL as hydrophilic emulsifier to stabilize the interfaces between the oily Montanide ISA 51 adjuvant and the antigen media. Methods: Polymers were synthesized by ring-opening polymerization of lactide and/or ε-caprolactone in the presence of monomethoxy PEG. 1H NMR and GPC data showed that obtained polymers consisted of 70 wt.% hydrophilic PEG block and 30 wt.% lipophilic PLA, PCL, PLACL block with molecular weights of 7,000. Results: The polymer-stabilized ISA51 emulsions have high affinity to water, such that the stock of antigen-encapsulating emulsion could be re-dispersed into PBS before injection, thus yielding stable and injectable W/O/W emulsion nanoparticles. Immunogenicity studies showed that PEG-b-PLACL/ISA51/PBS-formulated ovalbumin with only 5% of ISA51 oily adjuvant could induce the same level of antibody titers as those induced by PBS/ISA51-formulated ovalbumin. Conclusions: The novel multi-phase emulsions increase fluidity and conceptually diminish local reactions with respect to the W/O type vaccines produced from the same oil. These features are of great interest for applications in candidate vaccine delivery, especially for further optimization of alternative immunization routes, such as intramuscular, transdermal or mucosal administration.
Date:	2009-08
Relation:	Pharmaceutical Research. 2009 Aug;26(8):1856-1862.
Link to:	http://dx.doi.org/10.1007/s11095-009-9898-y
JIF/Ranking 2023:	http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0724-8741&DestApp=IC2JCR
Cited Times(WOS):	https://www.webofscience.com/wos/woscc/full-record/WOS:000267685800007
Cited Times(Scopus):	http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=67650032608
Appears in Collections:	[Pele Choi-Sing Chong] Periodical Articles [Chih-Hsiang Leng] Periodical Articles [Shih-Jen Liu] Periodical Articles [Hsin-Wei Chen] Periodical Articles [Ming-Hsi Huang] Periodical Articles

Files in This Item:

File	Description	Size	Format
SCP67650032608.pdf		267Kb	Adobe PDF	744	View/Open

Loading...