國家衛生研究院 NHRI:Item 3990099045/3602
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    Please use this identifier to cite or link to this item: http://ir.nhri.org.tw/handle/3990099045/3602


    Title: Genome sequencing and comparative analysis of Klebsiella pneumoniae NTUH-K2044, a strain causing liver abscess and meningitis
    Authors: Wu, KM;Li, LH;Yan, JJ;Tsao, N;Liao, TL;Tsai, HC;Fung, CP;Chen, HJ;Liu, YM;Wang, JT;Fang, CT;Chang, SC;Shu, HY;Liu, TT;Chen, YT;Shiau, YR;Lauderdale, TL;Su, IJ;Kirby, R;Tsai, SF
    Contributors: Division of Molecular and Genomic Medicine;Division of Clinical Research
    Abstract: Nosocomial infections caused by antibiotic-resistant Klebsiella pneumoniae are emerging as a major health problem worldwide, while community-acquired K. pneumoniae infections present with a range of diverse clinical pictures in different geographic areas. In particular, an invasive form of K. pneumoniae that causes liver abscesses was first observed in Asia and then was found worldwide. We are interested in how differences in gene content of the same species result in different diseases. Thus, we sequenced the whole genome of K. pneumoniae NTUH-K2044, which was isolated from a patient with liver abscess and meningitis, and analyzed differences compared to strain MGH 78578, which was isolated from a patient with pneumonia. Six major types of differences were found in gene clusters that included an integrative and conjugative element, clusters involved in citrate fermentation, lipopolysaccharide synthesis, and capsular polysaccharide synthesis, phage-related insertions, and a cluster containing fimbria-related genes. We also conducted comparative genomic hybridization with 15 K. pneumoniae isolates obtained from community-acquired or nosocomial infections using tiling probes for the NTUH-K2044 genome. Hierarchical clustering revealed three major groups of genomic insertion-deletion patterns that correlate with the strains' clinical features, antimicrobial susceptibilities, and virulence phenotypes with mice. Here we report the whole-genome sequence of K. pneumoniae NTUH-K2044 and describe evidence showing significant genomic diversity and sequence acquisition among K. pneumoniae pathogenic strains. Our findings support the hypothesis that these factors are responsible for the changes that have occurred in the disease profile over time.
    Date: 2009-07
    Relation: Journal of Bacteriology. 2009 Jul;191(14):4492-4501.
    Link to: http://dx.doi.org/10.1128/JB.00315-09
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0021-9193&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000267372900002
    Cited Times(Scopus): http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=67650324665
    Appears in Collections:[Shih-Feng Tsai] Periodical Articles
    [Ih-Jen Su(2002-2015)] Periodical Articles
    [Tsai-Ling Yang Lauderdale] Periodical Articles

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