國家衛生研究院 NHRI:Item 3990099045/3460
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    Please use this identifier to cite or link to this item: http://ir.nhri.org.tw/handle/3990099045/3460


    Title: One tube multiplex PCR for simple screening of SCCmec I-V types of methicillin-resistant Staphylococcus aureus
    Authors: Lu, PL;Chang, JC;Hsu, HT;Chen, JH;Chen, FJ;Lin, SF;Siu, LK
    Contributors: Division of Clinical Research
    Abstract: In this study, a rapid and simple one-tube multiplex PCR (M-PCR) method was developed for Staphylococcal Cassette Chromosome mec (SCCmec), primary screening. One hundred and eight methicillin-resistant Staphylococcus aureus (MRSA) isolates with SCC mec typing results using the Oliveira and de Lencastre method (Oliveira and de Lencastre. Antimicrob. Agents Chemother. 46:2155-2161, 2002), were evaluated for the efficacy of our method. Typing results were also compared to the recently published PCR protocol by Kondo et al., (Kondo et al., Antimicrob Agents Chemother. 51:264-74, 2007). The one-tube M-PCR method showed comparable efficacy to the other two methods. None of the isolates were classified as non-typable in Kondo's and our method. Two formerly assigned SCCmec type I isolates by Oliveira and de Lencastre were determined as SCCmec type IV by Kondo's and the method in this study. In conclusion, the one-tube M-PCR method developed in this study increased the rapidity and simplicity for primary screening SCCmec type I to V MRSA.
    Date: 2008-12
    Relation: Journal of Chemotherapy. 2008 Dec;20(6):690-696.
    Link to: http://www.jchemother.it/cgi-bin/digisuite.exe/product?ID=1849&IDCategory=144
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=1120-009X&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000262764100004
    Cited Times(Scopus): http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=59349101573
    Appears in Collections:[Leung-Kei Siu] Periodical Articles
    [Feng-Jui Chen] Periodical Articles

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