國家衛生研究院 NHRI:Item 3990099045/3404

NHRI > National Institute of Infectious Diseases and Vaccinology > Horng-Yunn Dou > Periodical Articles > Item 3990099045/3404

Please use this identifier to cite or link to this item: http://ir.nhri.org.tw/handle/3990099045/3404

Title:	Using a multiplex polymerase chain reaction for the identification of Beijing strains of Mycobacterium tuberculosis
Authors:	Sun, JR;Lee, SY;Dou, HY;Lu, JJ
Contributors:	Division of Clinical Research
Abstract:	The genotype of a Beijing strain of Mycobacterium tuberculosis (MTB) is usually determined by spoligotyping. However, this technique requires special equipment and is time-consuming. In this study, we developed a new multiplex polymerase chain reaction (PCR) to differentiate between Beijing and non-Beijing strains of MTB. A total of 323 MTB isolates were genotyped by both spoligotyping and the novel multiplex PCR. By spoligotyping, 169 (52.3%) isolates were determined to be Beijing strains and the remaining 154 (47.7%) isolates were non-Beijing strains. The multiplex PCR method produced results identical to those of spoligotyping in the identification of Beijing strains of MTB. This method is highly sensitive, specific, and fast. It is also cost-effective and suitable for screening large numbers of samples. ? 2008 Springer-Verlag.
Date:	2009-01
Relation:	European Journal of Clinical Microbiology and Infectious Diseases. 2009 Jan;28(1):105-107.
Link to:	http://dx.doi.org/10.1007/s10096-008-0590-7
JIF/Ranking 2023:	http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0934-9723&DestApp=IC2JCR
Cited Times(WOS):	https://www.webofscience.com/wos/woscc/full-record/WOS:000261968000017
Cited Times(Scopus):	http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=58149150039
Appears in Collections:	[Horng-Yunn Dou] Periodical Articles

Files in This Item:

File	Description	Size	Format
SCP58149150039.pdf		106Kb	Adobe PDF	620	View/Open

Loading...