國家衛生研究院 NHRI:Item 3990099045/3075

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Please use this identifier to cite or link to this item: http://ir.nhri.org.tw/handle/3990099045/3075

Title:	Bioassay-guided purification and identification of PPARa/gamma agonists from Chlorella sorokiniana
Authors:	Chou, YC;Prakash, E;Huang, CF;Lien, TW;Chen, X;Su, IJ;Chao, YS;Hsieh, HP;Hsu, JTA
Contributors:	Division of Biotechnology and Pharmaceutical Research;Division of Clinical Research
Abstract:	This study isolated agonists of peroxisome proliferator activated receptors (PPARs) from the green algae Chlorella sorokiniana, using a bioassay-guided purification strategy. PPARs are widely recognized as the molecular drug targets for many diseases including hyperglycemia, diabetes, obesity and cancer. Two independent bioassays were developed. The first is the scintillation proximity assay, a ligand binding assay. The other is the cell-based transcriptional activation assay which uses the Dual-Luciferase (R) reporter system as the reporter gene under the control of the PPAR response element. Using these two assays, a PPAR gamma-active fraction, CE 3-3, was obtained from C. sorokiniana extracts, which was also able to activate PPAR alpha-mediated gene expression. To elucidate the active ingredients in the CE 3-3 fraction, GC-MS analysis was employed. The results showed that the CE 3-3 fraction consisted of at least ten fatty acids (FAs). The bioactivities of several of the individual FAs were evaluated for their PPAR gamma activity and the results showed that linolenic acid and linoleic acid were the most potent FAs tested. Our studies indicate that Chlorella sorokiniana could have potential health benefits through the dual activation of PPAR alpha/gamma via its unique FA constituents.
Keywords:	Chemistry, Medicinal;Pharmacology & Pharmacy
Date:	2008-05
Relation:	Phytotherapy Research. 2008 May;22(5):605-613.
Link to:	http://dx.doi.org/10.1002/ptr.2280
JIF/Ranking 2023:	http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0951-418X&DestApp=IC2JCR
Cited Times(WOS):	https://www.webofscience.com/wos/woscc/full-record/WOS:000256128000006
Cited Times(Scopus):	http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=44149093347
Appears in Collections:	[John Tsu-An Hsu] Periodical Articles [Hsing-Pang Hsieh] Periodical Articles [Yu-Sheng Chao(2002-2013)] Periodical Articles [Xin Chen(2002-2015)] Periodical Articles [Ih-Jen Su(2002-2015)] Periodical Articles

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