國家衛生研究院 NHRI:Item 3990099045/3033
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    Please use this identifier to cite or link to this item: http://ir.nhri.org.tw/handle/3990099045/3033


    Title: Mice deficient in hepsin, a serine protease, exhibit normal embryogenesis and unchanged hepatocyte regeneration ability
    Authors: Yu, IS;Chen, HJ;Lee, YSE;Huang, PH;Lin, SR;Tsai, TW;Lin, SW
    Contributors: National Institute of Cancer Research
    Abstract: Hepsin, a liver-enriched novel serine protease, has been implicated in participating with normal cell growth, embryogenesis, and blood coagulation pathway. To study its function in vivo, we have disrupted the mouse hepsin gene by homologous recombination. Targeted disruption of the hepsin gene and ablation of hepsin message were demonstrated by Southern blotting, Northern blotting and RT-PCR analysis. Homozygous hepsin -/- mice were viable, fertile, and exhibited no gross abnormalities, as judged by the size, weight and blood coagulation (PT) assays. However, the serum concentration of the bone form of alkaline phosphatase, aspartate aminotransferase, and alanine aminotransferase of the hepsin -/- mice was mildly elevated, in spire of no obvious pathological change of hepatocytes, To examine whether hepsin is involved in controlling cell growth in adult tissues, 70% hepatectomy was applied to the hepsin -/- mice. Liver regeneration proceeded normally in the hepsin -/- mice as judged by the liver mass restoration rate. These results suggest that loss of hepsin function causes no effect in cell growth and embryogenesis in vivo, which is in contradiction to the studies using in vitro cell culturing system. Moreover, gross mass regeneration of liver after damage proceeds normally in the absence of functional hepsin.
    Keywords: Hematology;Peripheral Vascular Disease
    Date: 2000-11
    Relation: Thrombosis and Haemostasis. 2000 Nov;84(5):865-870.
    Link to: http://www.schattauer.de/index.php?id=1268&pii=th00110865&no_cache=1
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0340-6245&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000165403900023
    Cited Times(Scopus): http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0033724609
    Appears in Collections:[Others] Periodical Articles

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