國家衛生研究院 NHRI:Item 3990099045/3003
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    題名: Inverted repeats as genetic elements for promoting DNA inverted duplication: implications in gene amplification
    作者: Lin, CT;Lin, WH;Lyu, YL;Whang-Peng, J
    貢獻者: National Institute of Cancer Research
    摘要: Inverted repeats are important genetic elements for genome instability. In the current study we have investigated the role of inverted repeats in a DNA rearrangement reaction using a linear DNA substrate. We show that linear DNA substrates with terminal inverted repeats can efficiently transform Escherichia coli. The transformation products contain circular inverted dimers in which the DNA sequences between terminal inverted repeats are duplicated. In contrast to the recombination/rearrangement product of circular DNA substrates, which is exclusively one particular form of the inverted dimer, the rearrangement products of the linear DNA substrate consist of two isomeric forms of the inverted dimer. Escherichia coli mutants defective in RecBCD exhibit much reduced transformation efficiency, suggesting a role for RecBCD in the protection rather than destruction of these linear DNA substrates. These results suggest a model in which inverted repeats near the ends of a double-strand break can be processed by a helicase/exonuclease to form hairpin caps. Processing of hairpin capped DNA intermediates can then yield inverted duplications. Linear DNA substrates containing terminal inverted repeats can also be converted into inverted dimers in COS cells, suggesting conservation of this type of genome instability from bacteria to mammalian cells.
    關鍵詞: Biochemistry & Molecular Biology
    日期: 2001-09-01
    關聯: Nucleic Acids Research. 2001 Sep;29(17):3529-3538.
    Link to: http://dx.doi.org/10.1093/nar/29.17.3529
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0305-1048&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000171015500009
    Cited Times(Scopus): http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0035446529
    顯示於類別:[彭汪嘉康(1996-2007)] 期刊論文
    [林景太(1999-2007)] 期刊論文

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